Bioprospecting of potential seagrass Thalassia hemprichii (Ehrenb. ex solms) Asch. (hydrocharitaceae) extract from Pramuka Island against Aedes aegypti L. larvae

H. K. Purnomo, Windri Handayani, Yasman

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Citation (Scopus)

Abstract

Seagrass species have a high source of bioactive compounds and are potential to be developed in health and medicinal application. One of the seagrass species, Thalassia hemprichii, was collected from Pramuka Island, Kepulauan Seribu National Park, Jakarta. Samples were cut into different parts, i.e., leaf, rhizome, and root. Each part of the samples was dried as a powdered simplicia and extracted using methanol (1:3; w/v). All the extracts were characterized using High Performance Liquid Chromatography (HPLC) and tested as larvicide against the larvae of Aedes aegypti. Batches of 25 early 3rd instar larvae of Ae. aegypti were transferred into 250 mL sample bottles containing 1% extract of 100 mL distilled water. There are two control groups: abate (0.01%) and distilled water of each has the same volume as the treatments. The mortality of individual larvae was observed after 12, 24, and 48 hours. The leaf and root extract showed 100 % mortality while the rhizome extract showed 56 % mortality. There is no mortality of 3rd instar larvae of Ae. aegypti in the control sample bottle containing distilled water, while abate as the positive control showed 100 % mortality. Chromatogram results from 3 different parts of T. hemprichii showed a similar pattern of peaks except for the leaf extract that has an additional peak at Retention Time (RT) 5.877. The additional peak probably plays a role to enhance the larvicide activity and toxic to Ae. aegypti larvae. The results suggested that extract of T. hemprichii have the potential to be used as a larvicide against Ae. aegypti larvae.

Original languageEnglish
Title of host publicationProceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
EditorsRatna Yuniati, Terry Mart, Ivandini T. Anggraningrum, Djoko Triyono, Kiki A. Sugeng
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735417410
DOIs
Publication statusPublished - 22 Oct 2018
Event3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017 - Bali, Indonesia
Duration: 26 Jul 201727 Jul 2017

Publication series

NameAIP Conference Proceedings
Volume2023
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
Country/TerritoryIndonesia
CityBali
Period26/07/1727/07/17

Keywords

  • Aedes aegypti
  • extract, HPLC
  • Kepulauan Seribu National Park
  • larvicide
  • Thalassia hemprichii

Fingerprint

Dive into the research topics of 'Bioprospecting of potential seagrass Thalassia hemprichii (Ehrenb. ex solms) Asch. (hydrocharitaceae) extract from Pramuka Island against Aedes aegypti L. larvae'. Together they form a unique fingerprint.

Cite this