Bioprospect of lovastatin in Aspergillus spp. from University of Indonesia Culture Collection (UICC)

Wibowo Mangunwardoyo; Yanti Rafliyanti; Dadang Kusmana

Bioprospect of lovastatin in Aspergillus spp. from University of Indonesia Culture Collection (UICC)

Wibowo Mangunwardoyo, Yanti Rafliyanti, Dadang Kusmana

Department of Biology

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

The aim of this research is to select and analyse lovastatin from isolated molds of Aspergillus spp. from University of Indonesia Culture Collection (UICC) Lovastatin is an inhibitor 3 hydroxy-3-methylglutary-coenzyme-A reductase (HMG-CoA reductase) enzyme and a competitive inhibitor of the biosynthesis of cholesterol. The results revealed that out of 40 cultures, 18 cultures (45%) produced lovastatin and 22 cultures (55%) were negative. Aspergillus flavus UICC 360 showed the highest lovastatin production compared to a number of selected cultures. Thin Layer Chromatografy (TLC) analysis showed an amount of Aspergillus with same similatities of Rf value compared to the standard. High Performance Chromatography (HPLC) analysis which confirmed that lovastatin Aspergillus flavus UICC 360 has the same retention time with the standard (13.2 minutes).

Original language	English
Pages (from-to)	183-188
Number of pages	6
Journal	World Applied Sciences Journal
Volume	16
Issue number	2
Publication status	Published - 30 Jan 2012

Keywords

Aspergilllus spp
Extraction
HPLC
Lovastatin
Screening
TLC

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title = "Bioprospect of lovastatin in Aspergillus spp. from University of Indonesia Culture Collection (UICC)",

abstract = "The aim of this research is to select and analyse lovastatin from isolated molds of Aspergillus spp. from University of Indonesia Culture Collection (UICC) Lovastatin is an inhibitor 3 hydroxy-3-methylglutary-coenzyme-A reductase (HMG-CoA reductase) enzyme and a competitive inhibitor of the biosynthesis of cholesterol. The results revealed that out of 40 cultures, 18 cultures (45%) produced lovastatin and 22 cultures (55%) were negative. Aspergillus flavus UICC 360 showed the highest lovastatin production compared to a number of selected cultures. Thin Layer Chromatografy (TLC) analysis showed an amount of Aspergillus with same similatities of Rf value compared to the standard. High Performance Chromatography (HPLC) analysis which confirmed that lovastatin Aspergillus flavus UICC 360 has the same retention time with the standard (13.2 minutes).",

keywords = "Aspergilllus spp, Extraction, HPLC, Lovastatin, Screening, TLC",

author = "Wibowo Mangunwardoyo and Yanti Rafliyanti and Dadang Kusmana",

year = "2012",

month = jan,

day = "30",

language = "English",

volume = "16",

pages = "183--188",

journal = "World Applied Sciences Journal",

issn = "1818-4952",

publisher = "International Digital Organization for Scientific Information",

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TY - JOUR

T1 - Bioprospect of lovastatin in Aspergillus spp. from University of Indonesia Culture Collection (UICC)

AU - Mangunwardoyo, Wibowo

AU - Rafliyanti, Yanti

AU - Kusmana, Dadang

PY - 2012/1/30

Y1 - 2012/1/30

N2 - The aim of this research is to select and analyse lovastatin from isolated molds of Aspergillus spp. from University of Indonesia Culture Collection (UICC) Lovastatin is an inhibitor 3 hydroxy-3-methylglutary-coenzyme-A reductase (HMG-CoA reductase) enzyme and a competitive inhibitor of the biosynthesis of cholesterol. The results revealed that out of 40 cultures, 18 cultures (45%) produced lovastatin and 22 cultures (55%) were negative. Aspergillus flavus UICC 360 showed the highest lovastatin production compared to a number of selected cultures. Thin Layer Chromatografy (TLC) analysis showed an amount of Aspergillus with same similatities of Rf value compared to the standard. High Performance Chromatography (HPLC) analysis which confirmed that lovastatin Aspergillus flavus UICC 360 has the same retention time with the standard (13.2 minutes).

AB - The aim of this research is to select and analyse lovastatin from isolated molds of Aspergillus spp. from University of Indonesia Culture Collection (UICC) Lovastatin is an inhibitor 3 hydroxy-3-methylglutary-coenzyme-A reductase (HMG-CoA reductase) enzyme and a competitive inhibitor of the biosynthesis of cholesterol. The results revealed that out of 40 cultures, 18 cultures (45%) produced lovastatin and 22 cultures (55%) were negative. Aspergillus flavus UICC 360 showed the highest lovastatin production compared to a number of selected cultures. Thin Layer Chromatografy (TLC) analysis showed an amount of Aspergillus with same similatities of Rf value compared to the standard. High Performance Chromatography (HPLC) analysis which confirmed that lovastatin Aspergillus flavus UICC 360 has the same retention time with the standard (13.2 minutes).

KW - Aspergilllus spp

KW - Extraction

KW - HPLC

KW - Lovastatin

KW - Screening

KW - TLC

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