TY - JOUR
T1 - Biomaterial Characterization of Decellularized Human Amniotic Membrane Seeded with Fetal Human Cardiac Fibroblasts for Cardiac Tissue Engineering
AU - Azwani, Winda
AU - Cahya, Dini Aulia
AU - Gazali, Achmad Danny
AU - Karima, Amira Puti
AU - Oktavya, Galuh
AU - Istiqomah, Rahma Nur
AU - Bustami, Arleni
AU - Rizqy, Faiza Aisya
AU - Katili, Puspita Anggraini
AU - Purnama, Ujang
AU - Djer, Mulyadi M.
N1 - Publisher Copyright:
© 2025 Faculty of Engineering, Universitas Indonesia. All rights reserved.
PY - 2025/9/22
Y1 - 2025/9/22
N2 - The human amniotic membrane (hAM) is a promising biomaterial in cardiac tissue engineering known for excellent viability, anti-inflammatory properties, and ability to support cellular adhesion. Its potential as a biomaterial, particularly after decellularization, offers a novel approach for myocardial regeneration in conditions such as cardiomyopathy and heart failure. Therefore, this study aimed to characterize the ultrastructure of fetal human cardiac fibroblasts-decellularized hAM (fHCFs-dehAM) using scanning electron microscopy (SEM), identify the functional groups of dehAM through fourier transform infrared (FTIR) spectroscopy, assess vimentin expression in fHCFs-dehAM via immunocytochemistry, and evaluate fHCF cell proliferation to determine cell viability on dehAM. In the process, hAM was successfully decellularized using 0.2% (w/v) trypsin/0.25% (w/v) ethylenediaminetetraacetic acid (EDTA) in phosphate-buffered saline (PBS), confirmed by the removal of the native epithelial layer through hematoxylin-eosin (H&E) staining and ultrastructural analysis.
AB - The human amniotic membrane (hAM) is a promising biomaterial in cardiac tissue engineering known for excellent viability, anti-inflammatory properties, and ability to support cellular adhesion. Its potential as a biomaterial, particularly after decellularization, offers a novel approach for myocardial regeneration in conditions such as cardiomyopathy and heart failure. Therefore, this study aimed to characterize the ultrastructure of fetal human cardiac fibroblasts-decellularized hAM (fHCFs-dehAM) using scanning electron microscopy (SEM), identify the functional groups of dehAM through fourier transform infrared (FTIR) spectroscopy, assess vimentin expression in fHCFs-dehAM via immunocytochemistry, and evaluate fHCF cell proliferation to determine cell viability on dehAM. In the process, hAM was successfully decellularized using 0.2% (w/v) trypsin/0.25% (w/v) ethylenediaminetetraacetic acid (EDTA) in phosphate-buffered saline (PBS), confirmed by the removal of the native epithelial layer through hematoxylin-eosin (H&E) staining and ultrastructural analysis.
UR - https://www.scopus.com/pages/publications/105017064663
U2 - 10.14716/ijtech.v16i5.7808
DO - 10.14716/ijtech.v16i5.7808
M3 - Article
AN - SCOPUS:105017064663
SN - 2086-9614
VL - 16
SP - 1651
EP - 1664
JO - International Journal of Technology
JF - International Journal of Technology
IS - 5
ER -