BIOLOGICAL ACTIVITIES OF EXTRACTS AND SECONDARY METABOLITE OF Macaranga mappa BARK AS AN ANTIDIABETIC BY α-GLUCOSIDASE INHIBITOR, ANTIOXIDANT, AND CYTOTOXICITY

The biological activities of extracts and isolated compounds of Macaranga mappa stem bark for antidiabetic herbal medicine, antioxidant, and cytotoxicity activities have been determined. The antidiabetic, antioxidant, and cytotoxicity activities of the sample were carried out by inhibition value of α-glucosidase, DPPH method, and brine shrimp larvae method, respectively. The initial methanol extract and its fractions have good activities against antidiabetics and antioxidants, except for the hexane fraction. The fractions with the highest activity for the antidiabetic and antioxidant tests were the water fraction IC₅₀ 6.654 ppm and the butanol fraction IC₅₀ 32.34 ppm, respectively. The toxicity test results with the BSLT method showed that all fractions were non-toxic LC₅₀>1000 ppm. The isolated compound from the ethyl acetate fraction was a coumarin derivative, namely scopoletin, with antidiabetic and antioxidant activities.