The research was carried out in Guguak Sarai, West Sumatera, Indonesia. The purpose of this study was to document the diversity of medicinal plants by Minangkabau ethnic base on local knowledge. Data were collected using ethnobotanical approach through open-ended, semi-structured interview and exploration method. The sample consisted of 3 key informants and 94 respondents with provisions age ≥ 30 years old. Data were analyzed qualitatively using descriptive statistics. Total 158 medicinal plants species which belongs to 124 genera and 54 families were reported to be used in against 52 diseases. Among the diseases, gastrointestinal disorders had the highest number of medicinal plants to be used (37 species), skin diseases (36 species), postpartum cures (29 species), urinary tract disorders (26 species) and rheumatism (19 species). Fabaceae (Leguminosae) was the dominant families that used to treat the illness (12 species) followed by Euphorbiaceae, Lamiaceae, Poaceae (10 species) and Asteraceae (9 species).