Biodegradation of used lubricant oil hydrocarbons using Bacillus subtilis InaCC B289 and Pseudomonas aeruginosa InaCC B290 in single or mixed cultures

Dewi Rista Kurnia, Wibowo Mangunwardoyo, Hanies Ambarsari

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Biodegradation using bacteria is one of the alternative solutions in the management of environmental contamination by using the activity of living organisms. Bacillus subtilis and Pseudomonas aeruginosa can degrade hydrocarbons. Single and mixed cultures of B. subtilis InaCC B289 and P. aeruginosa InaCC B290 were selected for analysis of their capability to degrade hydrocarbons from used lubricant oil. Used lubricant oil at varied concentrations of 1%, 10%, 17%, and 20% were used as hydrocarbon sources. The parameters of dissolved oxygen (DO), pH, and optical density (OD) were measured. Analysis of hydrocarbon compound degradation was carried out using gas chromatography-mass spectrometry (GC-MS). The results of DO, pH, and OD were not significantly different (P > 0.05). The GC-MS results for P. aeruginosa InaCC B290 and mixed cultures suggest they are only capable of degrading low molecular weight hydrocarbons, while B. subtilis InaCC B289 is capable of degrading low and high molecular weight hydrocarbons with a total hydrocarbon reduction of 57.72%.

Original languageEnglish
Title of host publication8th Annual Basic Science International Conference
Subtitle of host publicationCoverage of Basic Sciences toward the World's Sustainability Challenges
EditorsCorina Karim, Rodliyati Azrianingsih, Mauludi Ariesto Pamungkas, Yoga Dwi Jatmiko, Anna Safitri
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735417397
DOIs
Publication statusPublished - 17 Oct 2018
Event8th Annual Basic Science International Conference: Coverage of Basic Sciences toward the World's Sustainability Challanges, BaSIC 2018 - Malang, East Java, Indonesia
Duration: 6 Mar 20187 Mar 2018

Publication series

NameAIP Conference Proceedings
Volume2021
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference8th Annual Basic Science International Conference: Coverage of Basic Sciences toward the World's Sustainability Challanges, BaSIC 2018
CountryIndonesia
CityMalang, East Java
Period6/03/187/03/18

Keywords

  • Bacillus subtilis InaCC B289
  • Pseudomonas aeruginosa InaCC B290
  • biodegradation
  • hydrocarbons
  • lubricant oil

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