TY - GEN
T1 - Biodegradation of used lubricant oil hydrocarbons using Bacillus subtilis InaCC B289 and Pseudomonas aeruginosa InaCC B290 in single or mixed cultures
AU - Kurnia, Dewi Rista
AU - Mangunwardoyo, Wibowo
AU - Ambarsari, Hanies
PY - 2018/10/17
Y1 - 2018/10/17
N2 - Biodegradation using bacteria is one of the alternative solutions in the management of environmental contamination by using the activity of living organisms. Bacillus subtilis and Pseudomonas aeruginosa can degrade hydrocarbons. Single and mixed cultures of B. subtilis InaCC B289 and P. aeruginosa InaCC B290 were selected for analysis of their capability to degrade hydrocarbons from used lubricant oil. Used lubricant oil at varied concentrations of 1%, 10%, 17%, and 20% were used as hydrocarbon sources. The parameters of dissolved oxygen (DO), pH, and optical density (OD) were measured. Analysis of hydrocarbon compound degradation was carried out using gas chromatography-mass spectrometry (GC-MS). The results of DO, pH, and OD were not significantly different (P > 0.05). The GC-MS results for P. aeruginosa InaCC B290 and mixed cultures suggest they are only capable of degrading low molecular weight hydrocarbons, while B. subtilis InaCC B289 is capable of degrading low and high molecular weight hydrocarbons with a total hydrocarbon reduction of 57.72%.
AB - Biodegradation using bacteria is one of the alternative solutions in the management of environmental contamination by using the activity of living organisms. Bacillus subtilis and Pseudomonas aeruginosa can degrade hydrocarbons. Single and mixed cultures of B. subtilis InaCC B289 and P. aeruginosa InaCC B290 were selected for analysis of their capability to degrade hydrocarbons from used lubricant oil. Used lubricant oil at varied concentrations of 1%, 10%, 17%, and 20% were used as hydrocarbon sources. The parameters of dissolved oxygen (DO), pH, and optical density (OD) were measured. Analysis of hydrocarbon compound degradation was carried out using gas chromatography-mass spectrometry (GC-MS). The results of DO, pH, and OD were not significantly different (P > 0.05). The GC-MS results for P. aeruginosa InaCC B290 and mixed cultures suggest they are only capable of degrading low molecular weight hydrocarbons, while B. subtilis InaCC B289 is capable of degrading low and high molecular weight hydrocarbons with a total hydrocarbon reduction of 57.72%.
KW - Bacillus subtilis InaCC B289
KW - Pseudomonas aeruginosa InaCC B290
KW - biodegradation
KW - hydrocarbons
KW - lubricant oil
UR - http://www.scopus.com/inward/record.url?scp=85056165053&partnerID=8YFLogxK
U2 - 10.1063/1.5062805
DO - 10.1063/1.5062805
M3 - Conference contribution
AN - SCOPUS:85056165053
T3 - AIP Conference Proceedings
BT - 8th Annual Basic Science International Conference
A2 - Karim, Corina
A2 - Azrianingsih, Rodliyati
A2 - Pamungkas, Mauludi Ariesto
A2 - Jatmiko, Yoga Dwi
A2 - Safitri, Anna
PB - American Institute of Physics Inc.
T2 - 8th Annual Basic Science International Conference: Coverage of Basic Sciences toward the World's Sustainability Challanges, BaSIC 2018
Y2 - 6 March 2018 through 7 March 2018
ER -