Biodegradation of diesel oil hydrocarbons using Bacillus subtilis InaCC B289 and Pseudomonas aeruginosa InaCC B290 in single and mixed cultures

Rina Marita Safitri, Wibowo Mangunwardoyo, Hanies Ambarsari

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Biodegradation is a method used to control diesel oil pollution, by utilizing bacteria to degrade the hydrocarbon compound. Bacillus subtilis and Pseudomonas aeruginosa are known to be able to degrade diesel oil. The objective of this study was to investigate the ability of both single and mixed cultures of B. subtilis InaCC B289 and P. aeruginosa InaCC B290 to degrade hydrocarbon compounds. Parameters were measured for 25 days and included pH, optical density (OD) and dissolved oxygen (DO). The results revealed that pH, OD, and DO were not significantly different (p > 0.05). Furthermore, the percentage of degradation was analyzed using gas chromatography-mass spectrometry (GC-MS) and showed that the degradation of the hydrocarbon compounds found in diesel oil was higher with mixed cultures of B. subtilis InaCC B289 and P. aeruginosa InaCCB290 (57.56%), compared to single cultures of B. subtilis InaCCB289 (37.53%) and P. aeruginosa InaCC B290 (36.50%).

Original languageEnglish
Title of host publication8th Annual Basic Science International Conference
Subtitle of host publicationCoverage of Basic Sciences toward the World's Sustainability Challenges
EditorsCorina Karim, Rodliyati Azrianingsih, Mauludi Ariesto Pamungkas, Yoga Dwi Jatmiko, Anna Safitri
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735417397
DOIs
Publication statusPublished - 17 Oct 2018
Event8th Annual Basic Science International Conference: Coverage of Basic Sciences toward the World's Sustainability Challanges, BaSIC 2018 - Malang, East Java, Indonesia
Duration: 6 Mar 20187 Mar 2018

Publication series

NameAIP Conference Proceedings
Volume2021
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference8th Annual Basic Science International Conference: Coverage of Basic Sciences toward the World's Sustainability Challanges, BaSIC 2018
CountryIndonesia
CityMalang, East Java
Period6/03/187/03/18

Keywords

  • Bacillus subtilis InaCC B289
  • Pseudomonas aeruginosa InaCC B290
  • biodegradation
  • diesel oil
  • hydrocarbon

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