TY - GEN
T1 - Biodegradation of diesel oil hydrocarbons using Bacillus subtilis InaCC B289 and Pseudomonas aeruginosa InaCC B290 in single and mixed cultures
AU - Safitri, Rina Marita
AU - Mangunwardoyo, Wibowo
AU - Ambarsari, Hanies
PY - 2018/10/17
Y1 - 2018/10/17
N2 - Biodegradation is a method used to control diesel oil pollution, by utilizing bacteria to degrade the hydrocarbon compound. Bacillus subtilis and Pseudomonas aeruginosa are known to be able to degrade diesel oil. The objective of this study was to investigate the ability of both single and mixed cultures of B. subtilis InaCC B289 and P. aeruginosa InaCC B290 to degrade hydrocarbon compounds. Parameters were measured for 25 days and included pH, optical density (OD) and dissolved oxygen (DO). The results revealed that pH, OD, and DO were not significantly different (p > 0.05). Furthermore, the percentage of degradation was analyzed using gas chromatography-mass spectrometry (GC-MS) and showed that the degradation of the hydrocarbon compounds found in diesel oil was higher with mixed cultures of B. subtilis InaCC B289 and P. aeruginosa InaCCB290 (57.56%), compared to single cultures of B. subtilis InaCCB289 (37.53%) and P. aeruginosa InaCC B290 (36.50%).
AB - Biodegradation is a method used to control diesel oil pollution, by utilizing bacteria to degrade the hydrocarbon compound. Bacillus subtilis and Pseudomonas aeruginosa are known to be able to degrade diesel oil. The objective of this study was to investigate the ability of both single and mixed cultures of B. subtilis InaCC B289 and P. aeruginosa InaCC B290 to degrade hydrocarbon compounds. Parameters were measured for 25 days and included pH, optical density (OD) and dissolved oxygen (DO). The results revealed that pH, OD, and DO were not significantly different (p > 0.05). Furthermore, the percentage of degradation was analyzed using gas chromatography-mass spectrometry (GC-MS) and showed that the degradation of the hydrocarbon compounds found in diesel oil was higher with mixed cultures of B. subtilis InaCC B289 and P. aeruginosa InaCCB290 (57.56%), compared to single cultures of B. subtilis InaCCB289 (37.53%) and P. aeruginosa InaCC B290 (36.50%).
KW - Bacillus subtilis InaCC B289
KW - Pseudomonas aeruginosa InaCC B290
KW - biodegradation
KW - diesel oil
KW - hydrocarbon
UR - http://www.scopus.com/inward/record.url?scp=85056154726&partnerID=8YFLogxK
U2 - 10.1063/1.5062737
DO - 10.1063/1.5062737
M3 - Conference contribution
AN - SCOPUS:85056154726
T3 - AIP Conference Proceedings
BT - 8th Annual Basic Science International Conference
A2 - Karim, Corina
A2 - Azrianingsih, Rodliyati
A2 - Pamungkas, Mauludi Ariesto
A2 - Jatmiko, Yoga Dwi
A2 - Safitri, Anna
PB - American Institute of Physics Inc.
T2 - 8th Annual Basic Science International Conference: Coverage of Basic Sciences toward the World's Sustainability Challanges, BaSIC 2018
Y2 - 6 March 2018 through 7 March 2018
ER -