Biodegradation of 0.02 % naphthalene added with 0.5 % glucose by Pseudomonas aeruginosa DRK 9.1 isolated from volcanic mud in Renokenongo Village, Sidoarjo

M. Fadilah, S. Sitaresmi, Y. Yasman, A. Oetari

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Naphthalene is a polycyclic aromatic hydrocarbon (PAH) pollutant that can be found in the environment. Naphthalene removal can be attained by biodegradation using bacteria. Volcanic mud contains organic materials including PAH, can support the growth of hydrocarbon degrading bacteria. Bacterial strain Pseudomonas aeruginosa DRK 9.1 was isolated from volcanic mud in Renokenongo Village, Sidoarjo. The objective of this research was to determine the capability of P. aeruginosa DRK 9.1 to degrade naphthalene with the addition of glucose as the growth stimulant. P. aeruginosa DRK 9.1 was grown in Bushnell-Haas medium with addition of 0.02 % (w/v) naphthalene and 0.5 % (w/v) glucose. Enumeration of the bacterial cells was performed using Total Plate Count (TPC) and quantitative determination of naphthalene degradation used High Performance Liquid Chromatography (HPLC). The results presented that the cell total number increased from 6.18 × 109 CFU/mL to 2.10 × 1011 CFU/mL after 24 h incubation. After 96 h incubation, the cell number decreased to 3.32 × 1010 CFU/mL. The results of naphthalene biodegradation by HPLC showed that naphthalene concentration decreased by 40.85 % and 82.47 % after 24 and 96 h incubation, respectively.

Original languageEnglish
Title of host publicationProceedings of the 5th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2019
EditorsTerry Mart, Djoko Triyono, Tribidasari Anggraningrum Ivandini
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735420014
DOIs
Publication statusPublished - 1 Jun 2020
Event5th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2019 - Depok, Indonesia
Duration: 9 Jul 201910 Jul 2019

Publication series

NameAIP Conference Proceedings
Volume2242
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference5th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2019
CountryIndonesia
CityDepok
Period9/07/1910/07/19

Keywords

  • Biodegradation
  • glucose
  • naphthalene
  • Pseudomonas aeruginosa
  • volcanic mud

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    Fadilah, M., Sitaresmi, S., Yasman, Y., & Oetari, A. (2020). Biodegradation of 0.02 % naphthalene added with 0.5 % glucose by Pseudomonas aeruginosa DRK 9.1 isolated from volcanic mud in Renokenongo Village, Sidoarjo. In T. Mart, D. Triyono, & T. A. Ivandini (Eds.), Proceedings of the 5th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2019 [050004] (AIP Conference Proceedings; Vol. 2242). American Institute of Physics Inc.. https://doi.org/10.1063/5.0008538