Bioactivity of Thalassia hemprichii (hydrocharitaceae) methanolic extract from West Bali National Park as Aedes aegypti larvacide

Y. Yusniawati, Yasman, Windri Handayani

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Citation (Scopus)

Abstract

Aedes aegypti mosquito is a vector of Dengue Hemorrhagic Fever (DHF) that caused by the dengue virus. To prevent dengue disease one possible way is through the termination of the transmission cycle by means of vector control like using chemical insecticides. This research tried to explore the potential of natural product from seagrass as larvicide and to characterize the compound from different origin of samples to see their larvicide activities. One of the seagrass species, Thalassia hemprichii obtained from Prapat Agung Beach and Menjangan Island, West Bali National Park. Samples were separated by organ, i.e., leaf, rhizome and roots, and their origin. The samples dried powdered and then extracted using methanol with ratio 1:3 (w/v). After that, the extract characterized using High Performance Liquid Chromatography (HPLC). The extract bioactivity as larvicide tested, batches of 25 early third instar larvaes of Ae. aegypti tested to 1 % concentration of each from total four extracts and two control group of abate (0.01 %) and distilled water, all have the same volume. The observation was done in 12, 24, and 48 hours, then the percentage of individual mortality counted. The entire samples have 100 % mortality percentage, but the leaves of Thalassia hemprichii from Menjangan Island has the highest effectiveness where all larvae died within 12 hours. HPLC chromatogram showed the compound of T. hemprichii leaves, rhizome and root from two different places relatively had the same pattern. However, there is a different peak in Thalassia hemprichii leaves at Retention Time (RT) 5.880, that peak considers as the compound responsible for the effectiveness of larvacidal activity.

Original languageEnglish
Title of host publicationProceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
EditorsRatna Yuniati, Terry Mart, Ivandini T. Anggraningrum, Djoko Triyono, Kiki A. Sugeng
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735417410
DOIs
Publication statusPublished - 22 Oct 2018
Event3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017 - Bali, Indonesia
Duration: 26 Jul 201727 Jul 2017

Publication series

NameAIP Conference Proceedings
Volume2023
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
Country/TerritoryIndonesia
CityBali
Period26/07/1727/07/17

Keywords

  • Aedes aegypti
  • HPLC
  • Thalassia hemprichii
  • larvicidal

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