BIOACTIVE COMPOUNDS OF FLAVONE DIMERS FROM INDONESIAN Araucaria columnaris LEAVES

This study aimed to isolate the flavone dimers in the acetone extract of Araucaria columnaris leaves and evaluate their potential biological activities. Three flavone dimers, 1-3, were isolated and identified by spectroscopic analysis using UV, IR, NMR, and mass spectrometry. The acetone extract and isolated metabolites were examined for their antiangiogenic activity against the Calf Pulmonary Arterial Endothelial (CPAE) cell and antioxidant performance using 2,2-diphenyl-1-picrylhydrazyl (DPPH). The results showed that 4',4'",7,7"-tetra-O-methylcupressuflavone (1) and acetone extract had IC₅₀ values of 272.95±7.05 and 239.36±13.50 μg/mL, respectively, against CPAE cell. The other two isolated metabolites, 4',7,7"-tri-O-methylcupressuflavone (2) and 4"',7-di-O-methylcupressuflavone (3), showed high activity with IC₅₀ values of 39.5±1.44 and 66.13±15.96 μg/mL, respectively. Although none of the individual components demonstrated any detectable antioxidant activity (IC₅₀ > 100 μg/mL), the acetone extract showed moderate antioxidant activity with an IC₅₀ value of 69.2 μg/mL.