Beta carotene protects against ischemic and reperfusion injury in the perfused rat liver

S. Ganiswarna, C. Vitasyana, Franciscus D. Suyatna, S. Pringgoutomo

Research output: Contribution to journalArticle

Abstract

This study was designed to investigate the effects of beta carotene on ischemic and reperfusion injury in rat liver perfused with a free-Ca++ Krebs-Henseleit buffer. The experimental animals were grouped as follows: C = control group perfused for 165 minutes, I = a group of livers rendered ischemic for 2 hours, IR = a group of livers rendered ischemic for 2 hours, followed by 30 min reperfusion, O = a group of ischemic and reperfused livers from rats pretreated with oral olive oil for 14 days, BI = a group of ischemic livers from rats pretreated with oral β carotene of 100 mg kg-1 b.w. daily in olive oil for 14 days, and B-IR = a group of ischemic and reperfused livers, pretreated with oral β carotene of 100 mg kg-1 b.w. daily in olive oil for 14 days. The results showed that an ischemic insult of 2 hours duration caused a significant increase of glutamic pyruvic transaminase (GPT) and glutamic oxalic transaminase (GOT) in the IR group as compared with control (GPT: 169 ± 40 U.L-1 vs 37 ± 20 U.L-1, p < 0.05 and GOT: 143 ± 19 U.L-1 vs 30 ± 10 U.L-1, p < 0.05, respectively), but thiobarbituric acid reactive substances (TBARS) contents was not significantly different from control (0.93 ± 0.29 nmol.L-1 vs 0.61 ± 0.11 nmol.L-1, p = 0.06). β Carotene protected against membrane injury caused by ischemia as revealed by lower activities of GPT in the BI group (116 ± 52 U.L-1) vs IR group (169 ± 40 U.L-1) and of GOT in the BI group (75 ± 19 U.L-1) vs the IR group (143 ± 19 U.L-1) (p < 0.05). Similar protective effects of β carotene were seen in the B-IR group vs the IR group (GPT: 113 ± 57 U.L-1 vs 169 ± 40 U.L-1, p < 0.05 and GOT: 68 ± 23 U.L-1 vs 143 ± 19 U.L-1, p < 0.05). After 2 hours of ischemia, the transaminase levels in B-IR group were significantly different from control (GPT: 113 ± 57 U.L-1 vs 37 ± 20 U.L-1, p < 0.05 and GOT: 68 ± 23 U.L-1 vs 30 ± 10 U.L-1, p < 0.05). The TBARS contents of the BI and B-IR groups (0.60 ± 0.18 nmol.L-1 and 0.61 ± 0.25 nmol.L-1, respectively) were not significantly different from control (0.61 ± 0.11 nmol.L-1), but were significantly lower than that of IR group (0.93 ± 0.29 nmol.L-1) (p < 0.05). Reperfusion of 30 min further increased the levels of GPT (IR : 433 ± 100 U.L-1 vs control: 124 ± 19 U.L-1, p < 0.05) and GOT : (IR: 390 ± 72 U.L-1 vs control: 120 ± 40 U.L-1, p < 0.05). β Carotene prevented the increase in GPT (B-IR: 179 ± 67 U.L-1 vs IR: 433 ± 100 U.L-1, p < 0.05), and in GOT (B-IR: 149 ± 78 U.L-1 vs IR: 390 ± 72 U.L-1, p < 0.05) and TBARS content (B-IR: 0.45 ± 0.01 nmol.L-1 vs IR: 1.83 ± 1.40 nmol.L-1, p > 0.05). This study demonstrated the protective effects of β carotene against ischemic and reperfusion damage in the liver.

Original languageEnglish
Pages (from-to)145-150
Number of pages6
JournalAsia Pacific Journal of Pharmacology
Volume13
Issue number4
Publication statusPublished - 1 Dec 1998

Keywords

  • β Carotene
  • Olive oil
  • Perfused rat liver
  • Reperfusion injury

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