TY - JOUR
T1 - Basic for Male Contraceptive Vaccine Development: Sperm Specific Antigen Production by Recombinant Protein Expression VDAC3
AU - Asmarinah,
AU - Susmiarsih, Tri Panjiasih
AU - Pujianto, Dwi Ari
AU - Winiati, Endang
AU - Malik, Amarila
PY - 2012
Y1 - 2012
N2 - Background: It has been known that Voltage Dependent Anion Channel isotipe 3 (VDAC3) play a role in sperm motility. This protein could be a target of immune-contraception development based-on sperm specific protein in the future. For this purpose, we would like to produce iniatialy sperm-specific VDAC3 recombinant protein. This study aimed to produce VDAC3 protein from pET 100 and 101/D-TOPO-VDAC3 recombinant in Escherichia coli strain BL21(DE3) bacteria which was induced by Isopropylthiogalactoside (IPTG).Method: The expresion of VDAC3 protein from pET 100 and 101/D-TOPO-VDAC3 recombinant construct in Escherichia coli strain BL21(DE3) bacteria was induced by variuos concentration from 0 to 1 mM of IPTG with incubation time of four hours. The expression of VDAC3 protein was measured by means of SDS-PAGE method.Result: VDAC3 recombinant protein expression was successfully induced by IPTG optimally inthe concentration of 0,6 mM during incubation time of four hours and showed by the existence of single band in the size of 20 kDa in the gel electrophoresis.Conclusion: Induction of IPTG to pET 100 and 101/D-TOPO-VDAC3 recombinant vectortranformed-Escherichia coli strain BL21(DE3) bacteria yield VDAC3 recombinant protein in the size of 20 kDa.
AB - Background: It has been known that Voltage Dependent Anion Channel isotipe 3 (VDAC3) play a role in sperm motility. This protein could be a target of immune-contraception development based-on sperm specific protein in the future. For this purpose, we would like to produce iniatialy sperm-specific VDAC3 recombinant protein. This study aimed to produce VDAC3 protein from pET 100 and 101/D-TOPO-VDAC3 recombinant in Escherichia coli strain BL21(DE3) bacteria which was induced by Isopropylthiogalactoside (IPTG).Method: The expresion of VDAC3 protein from pET 100 and 101/D-TOPO-VDAC3 recombinant construct in Escherichia coli strain BL21(DE3) bacteria was induced by variuos concentration from 0 to 1 mM of IPTG with incubation time of four hours. The expression of VDAC3 protein was measured by means of SDS-PAGE method.Result: VDAC3 recombinant protein expression was successfully induced by IPTG optimally inthe concentration of 0,6 mM during incubation time of four hours and showed by the existence of single band in the size of 20 kDa in the gel electrophoresis.Conclusion: Induction of IPTG to pET 100 and 101/D-TOPO-VDAC3 recombinant vectortranformed-Escherichia coli strain BL21(DE3) bacteria yield VDAC3 recombinant protein in the size of 20 kDa.
UR - http://indonesia.digitaljournals.org/index.php/idnmed/article/view/1211
M3 - Article
VL - 62
JO - Journal of the Indonesian Medical Association
JF - Journal of the Indonesian Medical Association
IS - 2
ER -
