Bacteriocin-like inhibitory substance (BLIS) activity of Streptococcus macedonicus MBF10-2 and its synergistic action in combination with antibiotics

Sharon Edith Grazia, Sumayyah Sumayyah, Fakhri Subhana Haiti, Muhamad Sahlan, Nicholas C.K. Heng, Amarila Malik

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Objective To characterize the bacteriocin-like inhibitory-substances (BLIS) activity of Streptococcus macedonicus MBF10-2 [named BLIS(MBF10-2)], a bacteriocinogenic strain isolated from an Indonesian tofu byproduct. Methods BLIS(MBF10-2) was obtained by culturing the bacterium, and standard deferred antagonism assays were used to demonstrate its activity. The antibacterial testing of fractions collected by filtration using 3–30 kDa cut-off membrane sizes were carried out by performing well diffusion method. Results The growth of Micrococcus luteus, Streptococcus pyogenes, Lactococcus lactis, Leuconostoc mesenteroides and Weissella confusa were inhibited by BLIS(MBF10-2). Interestingly, BLIS-containing fractions obtained from sequential application on ultrafiltration membranes indicated that this bacterium Streptococcus macedonicus MBF10-2 could produces at least two antimicrobial peptides activities, one of which is likely to be a lantibiotic peptide. Potential synergistic activity against certain Gram-positive (but not Gram-negative) species when partnered with antibiotics (ampicillin, tetracycline or kanamycin) were observed. Conclusion Combination of some BLIS(MBF10-2) active fractions with antibiotics (ampicillin, tetracycline or kanamycin) could demonstrate synergistic activities against certain Gram-positive species.

Original languageEnglish
Pages (from-to)1140-1145
Number of pages6
JournalAsian Pacific Journal of Tropical Medicine
Volume10
Issue number12
DOIs
Publication statusPublished - 1 Dec 2017

Keywords

  • Bacteriocin-like inhibitory substance
  • Deferred antagonism assay
  • Lantibiotic
  • Protein fractionation
  • Streptococcus macedonicus
  • Well diffusion assay

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