Currently, manual tools such as rulers or calipers have been used to measure the diameter of the inhibition zone of an antimicrobial. The inhibition zone is the main indicator of an antimicrobial effectiveness. The current camera technology supports for a virtual instrumentation application in measuring the dimension of an object. The presence of another supporting device such as light sources, causing the camera is not an upright position to the measured object. In this study, an automatic tilting correction system is introduced using the low-cost camera to measure the dimension of the inhibition zones in the flexible tilting angle. The hardware of the system consists of a set of the tilt measuring instruments, a source light and a camera that connected to PC. The software consists of image processing techniques performing camera calibration, spatial calibration, geometrical transformations and transforming pixel value to a meter value. The experiments have been conducted using standard checkerboard calibrator objects and inhibition zone objects at various orientations and the angle of rotation of the object. Tilt correction system improves the measurement system performance with a reduction in estimated errors up to 39,6%. The maximum angle with a tolerance level that is acceptable is 40°. The system has been successfully implemented and could be used as an instrument for antimicrobial effectiveness evaluation in flexible tilting position.