Autologous microlobular fat combined with platelet-rich fibrin is associated with good fat graft viability

M. H. Reksodiputro, D. Diandini, T. Koento, R. Arisanty, A. R. Harahap

Research output: Contribution to journalConference articlepeer-review

2 Citations (Scopus)


Fat grafting is popular in facial plastic reconstructive surgery. However, a fat graft shows unpredictable resorption. The addition of autologous platelet-rich fibrin (PRF) may enhance its viability. PRF has several growth factors, including vascular endothelial growth factor (VEGF), and these factors can improve fat graft viability by enhancing angiogenesis. We aimed to determine whether the use of PRF and the graft processing method influence fat graft viability (angiogenesis and VEGF expression). The study included New Zealand white rabbits. The rabbits were divided into the following groups: microlobular fat (A), microlobular fat with PRF (B), centrifuged fat (C), and centrifuged fat with PRF (D). After 30 days, biopsy was performed for all grafts. Hematoxylin and eosin staining and immunohistochemical staining for VEGF were performed. The angiogenesis areas in groups A to D were 112.67, 90.51, 84.48, and 110.52, respectively. The VEGF expressions in groups A to D were 241.33, 287.22, 212.89, and 231.44, respectively. Increased angiogenesis and VEGF expression can improve graft viability. PRF was found to be associated with increased VEGF expression, whereas microlobular fat was found to be associated with good angiogenesis. Thus, microlobular fat combined with PRF is recommended as an autologous graft.

Original languageEnglish
Article number032060
JournalJournal of Physics: Conference Series
Issue number3
Publication statusPublished - 7 Sept 2018
Event2nd Physics and Technologies in Medicine and Dentistry Symposium, PTMDS 2018 - Depok, West Java, Indonesia
Duration: 18 Jul 201818 Jul 2018


Dive into the research topics of 'Autologous microlobular fat combined with platelet-rich fibrin is associated with good fat graft viability'. Together they form a unique fingerprint.

Cite this