TY - JOUR
T1 - Attenuation of cisplatin-induced hepatotoxicity by nanocurcumin through modulation of antioxidative and anti-inflammatory pathways
AU - Louisa, Melva
AU - Putera, Azis M.
AU - Sidqi, Antasena A.
AU - Mahaputra, Dimas Kirana
AU - Firmansyah, Ekida R.
AU - Ammar, Muhammad F.
AU - Talya, Natasha
AU - Aryadevi, Ni Nyoman Berlian
AU - Sandhiutami, Ni Made Dwi
AU - Sukmawati, Dewi
AU - Soetikno, Vivian
N1 - Funding Information:
This work was funded by Universitas Indonesia grants (Contract No. NKB-1293/UN2.RST/PPM.00.03.01/2020).
Publisher Copyright:
© 2023 Melva Louisa et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/)
PY - 2023
Y1 - 2023
N2 - The present study aimed to investigate whether curcumin in nanoparticle form may provide hepatoprotection in an ovarian cancer model in rats treated with cisplatin. Twenty-five female Wistar rats were divided into five 4-week treatment groups: (1) healthy rat group and four groups of 7,12-dimethylbenz(a)anthracene-induced ovarian cancer model rats receiving (2) vehicle only, (3) cisplatin 4 mg/kg BW/week, (4) cisplatin 4 mg/kg BW/week + curcumin 100 mg/kg BW/day, and (5) cisplatin 4 mg/kg BW/week + nanocurcumin (NC) 100 mg/kg BW/day. At the end of the treatment, a histopathological evaluation was carried out on the liver samples, in addition to oxidative stress, apoptosis, inflammatory, and fibrosis markers analyses. The group treated with cisplatin + NC had lower aspartate transaminase and alanine transaminase levels and lowered malondialdehyde and higher glutathione levels than those treated with cisplatin only. In addition, the nuclear factor-erythroid factor 2-related factor 2/Kelch-like ECH-associated protein 1 pathway genes were upregulated in the group treated with cisplatin + NC. We also demonstrated that NC effectively suppressed the gene expression of inflammatory marker tumor necrosis factor-α, as well as fibrosis marker transforming growth factor-β1. Furthermore, NC treatment reduced the fibrotic area in the hepatic tissues, as shown by Masson’s trichrome staining.
AB - The present study aimed to investigate whether curcumin in nanoparticle form may provide hepatoprotection in an ovarian cancer model in rats treated with cisplatin. Twenty-five female Wistar rats were divided into five 4-week treatment groups: (1) healthy rat group and four groups of 7,12-dimethylbenz(a)anthracene-induced ovarian cancer model rats receiving (2) vehicle only, (3) cisplatin 4 mg/kg BW/week, (4) cisplatin 4 mg/kg BW/week + curcumin 100 mg/kg BW/day, and (5) cisplatin 4 mg/kg BW/week + nanocurcumin (NC) 100 mg/kg BW/day. At the end of the treatment, a histopathological evaluation was carried out on the liver samples, in addition to oxidative stress, apoptosis, inflammatory, and fibrosis markers analyses. The group treated with cisplatin + NC had lower aspartate transaminase and alanine transaminase levels and lowered malondialdehyde and higher glutathione levels than those treated with cisplatin only. In addition, the nuclear factor-erythroid factor 2-related factor 2/Kelch-like ECH-associated protein 1 pathway genes were upregulated in the group treated with cisplatin + NC. We also demonstrated that NC effectively suppressed the gene expression of inflammatory marker tumor necrosis factor-α, as well as fibrosis marker transforming growth factor-β1. Furthermore, NC treatment reduced the fibrotic area in the hepatic tissues, as shown by Masson’s trichrome staining.
KW - Chronic liver injury
KW - Keap1
KW - nanoparticles
KW - Nrf2
KW - oxidative damage
KW - turmeric
UR - http://www.scopus.com/inward/record.url?scp=85152034643&partnerID=8YFLogxK
U2 - 10.7324/JAPS.2023.44149
DO - 10.7324/JAPS.2023.44149
M3 - Article
AN - SCOPUS:85152034643
SN - 2231-3354
VL - 13
SP - 60
EP - 70
JO - Journal of Applied Pharmaceutical Science
JF - Journal of Applied Pharmaceutical Science
IS - 3
ER -