Association of IL-8 −251 A/T Polymorphism and Osteoporosis in Postmenopausal Indonesian Women

Osteoporosis is a common bone disease known to have a strong genetic component, but the details of the traits and underlying mechanisms remain poorly understood. In postmenopausal women, osteoporosis is characterized by decreased bone mineral density. Interleukin-8 (IL-8) is an inflammatory marker, commonly detected in postmenopausal women with osteoporosis. This cytokine has been suggested to play a role in bone resorption, and is potentially influenced by polymorphisms of the encoding (IL-8) gene. The study aimed to investigate the association of the −251 A/T polymorphism of IL-8 with the risk of osteoporosis in postmenopausal Indonesian women. Using polymerase chain reaction-restriction fragment length polymorphism analysis, we assessed the status of the IL-8 −251 A/T polymorphism in stored DNA samples of 75 postmenopausal women with osteoporosis and of 25 healthy control subjects. Genotype and allotype comparisons were carried out according to T-score grouping. Chi-Square analysis revealed that subjects with AT and TT genotypes of the IL-8 −251 A/T polymorphism were at a significant risk of osteoporosis (odds ratio = 4.540, 95%). The results indicate that the −251 A/T polymorphism of IL-8 is significantly associated with osteoporosis in the tested Indonesian sample population.