TY - JOUR
T1 - Association of Gene Expression of Porphyromonas Gingivalis with Cigarette Smoking and Periodontal Pocket Depth
AU - Laksana, Valeo Adika
AU - Masulili, Sri Lelyati C.
AU - Sunarto, Hari
AU - Bachtiar, Boy M.
N1 - Funding Information:
This study was supported by the Faculty of Dentistry Universitas Indonesia and was funded by a 2019 PITTA B grant (Hibah PITTA 2019), Universitas Indonesia. The publication of this manuscript is supported by Universitas Indonesia.
Publisher Copyright:
© 2020. All Rights Reserved.
PY - 2020
Y1 - 2020
N2 - Periodontal disease is a complex disease that involves many bacteria including Porphyromonas gingivalis (P. gingivalis) in its pathophysiological process. We sought to analyze and correlate levels of P. gingivalis based on smoking status and periodontal pocket depth in periodontitis subjects. This was a cross-sectional study of smokers aged 35 to 60 years with periodontitis. Clinical data (periodontal pocket depth), smoking status information (pack-years), and Gingival Crevicular Fluid (GCF) samples were collected. The gene expression of P. gingivalis in GCF was measured by quantitative real-time polymerase chain reaction (qPCR). Spearman correlation testing between P. Igingivalis in GCF and periodontal pocket depth and smoking status was completed. P. gingivalis findings in shallow pocket versus deep pocket populations (P=0.72) and nonsmokers, moderate smokers, and heavy smokers (P=0.258) showed no significant difference. No significant correlation was present between P. gingivalis proportion and periodontal pocket depth (r=0.128; P=0.552), while the correlation between P. gingivalis and smoking status was moderate but statistically insignificant (r=0.488; P=0.016). Periodontal pocket depth and smoking status are not primary factors influencing the periodontal microflora, although variable gene expression of P. gingivalis was observed between groups.
AB - Periodontal disease is a complex disease that involves many bacteria including Porphyromonas gingivalis (P. gingivalis) in its pathophysiological process. We sought to analyze and correlate levels of P. gingivalis based on smoking status and periodontal pocket depth in periodontitis subjects. This was a cross-sectional study of smokers aged 35 to 60 years with periodontitis. Clinical data (periodontal pocket depth), smoking status information (pack-years), and Gingival Crevicular Fluid (GCF) samples were collected. The gene expression of P. gingivalis in GCF was measured by quantitative real-time polymerase chain reaction (qPCR). Spearman correlation testing between P. Igingivalis in GCF and periodontal pocket depth and smoking status was completed. P. gingivalis findings in shallow pocket versus deep pocket populations (P=0.72) and nonsmokers, moderate smokers, and heavy smokers (P=0.258) showed no significant difference. No significant correlation was present between P. gingivalis proportion and periodontal pocket depth (r=0.128; P=0.552), while the correlation between P. gingivalis and smoking status was moderate but statistically insignificant (r=0.488; P=0.016). Periodontal pocket depth and smoking status are not primary factors influencing the periodontal microflora, although variable gene expression of P. gingivalis was observed between groups.
KW - Adult
KW - Cross-sectional studies
KW - Real-time polymerase chain reaction
UR - http://www.scopus.com/inward/record.url?scp=85103513262&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85103513262
SN - 1309-100X
VL - 13
SP - 1370
EP - 1375
JO - Journal of International Dental and Medical Research
JF - Journal of International Dental and Medical Research
IS - 4
ER -