Association of Chlamydia trachomatis, Mycoplasma spp., Ureaplasma urealyticum and U. parvum with Human Papillomavirus in Patients with Cervical Cancer

Besides human papillomavirus (HPV), the cervical carcinogenesis is also affected by many risk factors including pathogenic bacteria such as Chlamydia trachomatis (CT), Mycoplasma spp. (MS), Ureaplasma urealyticum (UU), and U. parvum (UP) infections. Thus, we studied the bacterial infections for 68 patients with cervical cancerous cases and other cervical problems. Cervical swab samples were collected by specialist doctors and tested for the pathogenic bacteria by real time polymerase chain reaction (rPCR) and HPV by conventional PCR. Of 68 patients, 22 were diagnosed as cervical cancer and 46 were diagnosed as other cervical problems. All patients with cervical cancer were HPV positive, while the patients with other cervical problems were HPV negative. Of 22 HPV positive-cervical cancer patients, 7 (31.82%), 6 (27.27%), 3 (13.64%) were positive for MS, UU, and UP, respectively. None of the patients was CT positive. For 46 HPV negative-other cervical problem patients, 6 (13.04%), 12 (26.09%), 20 (43.48%), and 22 (47.83%) were positive for CT, MS, UU, and UP, respectively. There was no association of CT, MS, and UU infections with the HPV positive-cervical cancer patients (p >0.05). However, there is a negative correlation between UP infection and the HPV negative-other cervical problem patients (p<0.05).