TY - JOUR
T1 - Association between Volatile Sulfur Compounds Prevotella intermedi a and Matrix Metalloproteinase-8 Expression
AU - Maitimu, Fergy Christin
AU - Soeroso, Yuniarti
AU - Sunarto, Hari
AU - Bachtiar, Boy Muchlis
N1 - Funding Information:
This study was sponsored by Competitive Grant of International Final Indexed Publication: “HIBAH SKEMA PITTA B” promoted by Directorate of Research and Community Engagement of Universitas Indonesia, Jakarta – Indonesia.
Publisher Copyright:
© 2022, Association of Support to Oral Health Research (APESB). All rights reserved.
PY - 2022/1/24
Y1 - 2022/1/24
N2 - Objective: To determine the correlation between levels of methyl mercaptan (CH3SH) hydrogen sulfide (H2S), the proportion of Prevotella intermedia (Pi), and matrix metalloproteinase-8 (MMP-8) gene expression levels in periodontitis patients accompanied by halitosis. Material and Methods: Samples were obtained from gingival crevicular fluid (GCF) in the deepest pocket and by swabbing in the tongue coating area in patients with periodontitis presenting with halitosis (n = 23) and healthy subjects as controls (n = 7). The values of CH3SH and H2S were obtained using Oral Chroma. The proportion of Pi and MMP-8 expression levels were evaluated using PCR-RT. All the result was statistically analyzed using SPSS software. Results: The levels of CH3SH and H2S in participants with PD ≥ 6 mm showed a robust negative correlation with the proportion of P. intermedia in GCF and tongue coating. No statistically significant association was detected between CH3SH and H2S levels and MMP-8 expression levels (p>0.05). Conclusion: There is no association between CH3SH and H2S levels, the proportion of P. intermedia, and MMP-8 expression in patients with periodontitis accompanied by halitosis.
AB - Objective: To determine the correlation between levels of methyl mercaptan (CH3SH) hydrogen sulfide (H2S), the proportion of Prevotella intermedia (Pi), and matrix metalloproteinase-8 (MMP-8) gene expression levels in periodontitis patients accompanied by halitosis. Material and Methods: Samples were obtained from gingival crevicular fluid (GCF) in the deepest pocket and by swabbing in the tongue coating area in patients with periodontitis presenting with halitosis (n = 23) and healthy subjects as controls (n = 7). The values of CH3SH and H2S were obtained using Oral Chroma. The proportion of Pi and MMP-8 expression levels were evaluated using PCR-RT. All the result was statistically analyzed using SPSS software. Results: The levels of CH3SH and H2S in participants with PD ≥ 6 mm showed a robust negative correlation with the proportion of P. intermedia in GCF and tongue coating. No statistically significant association was detected between CH3SH and H2S levels and MMP-8 expression levels (p>0.05). Conclusion: There is no association between CH3SH and H2S levels, the proportion of P. intermedia, and MMP-8 expression in patients with periodontitis accompanied by halitosis.
KW - Halitosis
KW - Matrix Metalloproteinase-8
KW - Periodontitis
KW - Prevotella intermedia
UR - http://www.scopus.com/inward/record.url?scp=85139064852&partnerID=8YFLogxK
U2 - 10.1590/pboci.2022.049
DO - 10.1590/pboci.2022.049
M3 - Article
AN - SCOPUS:85139064852
SN - 1519-0501
VL - 22
JO - Pesquisa Brasileira em Odontopediatria e Clinica Integrada
JF - Pesquisa Brasileira em Odontopediatria e Clinica Integrada
M1 - e210002
ER -