Objective. This study aims to evaluate the level of male participation and factors associated with male participation in antenatal care. Methods. A cross-sectional study was performed, involving a survey of 381 men, selected through multistage random sampling. The outcome variable male participation in antenatal care was constructed from eight dichotomized indicators, and measurement results were low (scored 1 and 2) and high (scored 3 and 4). Multiple logistic regression analysis was performed using SPSS 24.0 at a significance level of 0.05. Results. The percentage of male participation in antenatal care was low (41.2%). Associated factors included age (OR=1.858, 95%CI=1.066-3.240), number of children (OR=2.909, 95%CI=1.532-5.522), income (OR=1.715, 95%CI=1.060-2.775), and knowledge (OR=3.706, 95%CI=2.320-5.919). Knowledge was found to be the main factor for male participation in antenatal care in Muaro Jambi Regency. Conclusion. Male participation in antenatal care in Muaro Jambi District was low and was influenced by age, number of children, income, and knowledge. Health promotion programs are needed to empower men to participate in antenatal care by providing communication, education, and information.