Assessment method for bacterial virulence based on ultraviolet and visible (UV-vis) spectroscopy analysis

Cholid Badri, Budiman Bella, Anwar Soefi Ibrahim, Sutarmo Setiadji

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Purpose: To develop assessment method of bacterial virulence based on UV-Vis spectroscopy analysis. The method was based on the measurement of absorbance of DNA extracted from the bacterial cultures during the growth phases. Methods: Non-pathogenic E. coli samples were taken from bacterial cultures in the exponential phase as well as the stationary phase of the bacteria. DNA extraction from the bacteria in growth phases were measured their normalized optical density (OD) at UV-Vis spectroscopy in the range of 240 - 320 nm. The same procedures were performed with pathogenic E. coli. Therefore, four groups containing 15 samples in each group were measured of their OD. Results: In non-pathogenic E. coli, the optical densities at the exponential phase were significantly lower as compared with the stationary phase, while in pathogenic E. coli the optical densities at the exponential phase were not significantly different than the stationary phase. In non-pathogenic E. coli groups, the optical densities at the exponential phase were significantly lower than in the corresponding phase in pathogenic E. coli, while the optical densities at the stationary phase of non-pathogenic E. coli were significantly lower than in the correspondence phase of pathogenic E. coli. Conclusion: DNA quantities of pathogenic E. coli were significantly higher than in non-pathogenic E. coli in the both exponential as well as stationary phases while DNA quantities of pathogenic - E. coli in the exponential phases were not different than in the stationary phase. Therefore the pattern of DNA quantities in growth phases could be considered as virulence indicator for E. coli. This method could be adopted as an alternative to other methods used for determination of bacterial virulence.

Original languageEnglish
Title of host publication7th WACBE World Congress on Bioengineering, 2015
EditorsChwee Teck Lim, James Goh
PublisherSpringer Verlag
Pages15-18
Number of pages4
ISBN (Print)9783319194516
DOIs
Publication statusPublished - 1 Jan 2015
Event7th World Congress on Bioengineering, WACBE 2015 - Kent Ridge, Singapore
Duration: 6 Jul 20158 Jul 2015

Publication series

NameIFMBE Proceedings
Volume52
ISSN (Print)1680-0737

Conference

Conference7th World Congress on Bioengineering, WACBE 2015
CountrySingapore
CityKent Ridge
Period6/07/158/07/15

Keywords

  • Bacterial virulence
  • DNA quantitative analysis
  • Escherichia coli
  • Optical density
  • UV-Vis Spectroscopy

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    Badri, C., Bella, B., Ibrahim, A. S., & Setiadji, S. (2015). Assessment method for bacterial virulence based on ultraviolet and visible (UV-vis) spectroscopy analysis. In C. T. Lim, & J. Goh (Eds.), 7th WACBE World Congress on Bioengineering, 2015 (pp. 15-18). (IFMBE Proceedings; Vol. 52). Springer Verlag. https://doi.org/10.1007/978-3-319-19452-3_5