TY - GEN
T1 - Assessing carbon availability in bioelectrochemical systems for nitrate removal by environmental isolates
AU - Ekadewi, Putty
AU - Putri, Khusnul Layli
AU - Pratiwi, Rizka Diva
AU - Arbianti, Rita
AU - Utami, Tania Surya
AU - Hermansyah, Heri
N1 - Publisher Copyright:
© 2020 Author(s).
PY - 2020/9/3
Y1 - 2020/9/3
N2 - Nitrate (NO3-) is one of the most common targets for water quality control. High nitrate concentration in the water systems negatively impact human health and the environment. Excessive nitrate content in the aquatic environment led to a decrease in water quality, which can be evaluated from changes in color, odor, increased turbidity, and reduced levels of dissolved oxygen. Denitrification as a biological method of tackling nitrate pollution has been studied since three decades ago in various systems, including recent investigations into bioelectrochemical systems. Bioelectrochemical cells are driven by microorganisms in carrying out oxidation/reduction reactions such as denitrification. However, the popularity of BES is still low. This study aim to optimize the source of carbon as substrate in culture media for the system and compare the efficiency of nitrate elimination and denitrification rate among isolates and culture conditions. Axenic cultures of Pseudomonas entomophila and Acinetobacter oleivorans were done in 20 mL single-chambered Microbial Electrolysis Cells (MECs) equipped with graphite as anode and stainless steel mesh as cathode. Sodium acetate and sodium bicarbonate was used as carbon sources in the formulation of heterotrophic and mixotrophic media. Denitrification performance in bioelectrochemical cells was evaluated from the evolution of nitrate concentration in the medium and the growth of target microorganisms. Environmental isolates presented similar behavior on the medium supplemented with bicarbonate by reaching 68-69% maximum nitrate removal efficiency across all experiments, suggesting inability to carry out autotrophic denitrification. On the other hand, Pseudomonas aeruginosa reached 78% efficiency on acetate-only medium, suggesting a preference towards acetate as carbon source over bicarbonate. Environmental isolates presented an interesting potential of tolerating the potential inhibitory effects of sodium bicarbonate in the medium compared to Pseudomonas aeruginosa, which demonstrated inhibitory effects in this medium.
AB - Nitrate (NO3-) is one of the most common targets for water quality control. High nitrate concentration in the water systems negatively impact human health and the environment. Excessive nitrate content in the aquatic environment led to a decrease in water quality, which can be evaluated from changes in color, odor, increased turbidity, and reduced levels of dissolved oxygen. Denitrification as a biological method of tackling nitrate pollution has been studied since three decades ago in various systems, including recent investigations into bioelectrochemical systems. Bioelectrochemical cells are driven by microorganisms in carrying out oxidation/reduction reactions such as denitrification. However, the popularity of BES is still low. This study aim to optimize the source of carbon as substrate in culture media for the system and compare the efficiency of nitrate elimination and denitrification rate among isolates and culture conditions. Axenic cultures of Pseudomonas entomophila and Acinetobacter oleivorans were done in 20 mL single-chambered Microbial Electrolysis Cells (MECs) equipped with graphite as anode and stainless steel mesh as cathode. Sodium acetate and sodium bicarbonate was used as carbon sources in the formulation of heterotrophic and mixotrophic media. Denitrification performance in bioelectrochemical cells was evaluated from the evolution of nitrate concentration in the medium and the growth of target microorganisms. Environmental isolates presented similar behavior on the medium supplemented with bicarbonate by reaching 68-69% maximum nitrate removal efficiency across all experiments, suggesting inability to carry out autotrophic denitrification. On the other hand, Pseudomonas aeruginosa reached 78% efficiency on acetate-only medium, suggesting a preference towards acetate as carbon source over bicarbonate. Environmental isolates presented an interesting potential of tolerating the potential inhibitory effects of sodium bicarbonate in the medium compared to Pseudomonas aeruginosa, which demonstrated inhibitory effects in this medium.
UR - http://www.scopus.com/inward/record.url?scp=85092046045&partnerID=8YFLogxK
U2 - 10.1063/5.0013694
DO - 10.1063/5.0013694
M3 - Conference contribution
AN - SCOPUS:85092046045
T3 - AIP Conference Proceedings
BT - 4th International Tropical Renewable Energy Conference, i-TREC 2019
A2 - Kusrini, Eny
A2 - Nugraha, I. Gde Dharma
PB - American Institute of Physics Inc.
T2 - 4th International Tropical Renewable Energy Conference 2019, i-TREC 2019
Y2 - 14 August 2019 through 16 August 2019
ER -
