Asaccharolytic anaerobic gram-negative coccobacilli (AAGNC) isolated from infected root canals and periodontal pockets

Ariadna Adisattya Djais, F. Nakazawa, M. Sato, N. Sato, G. Sundqvist, E. Hoshino

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Background/aims: Culture-difficult bacteria, including asaccharolytic anaerobic gram-negative coccobacilli (AAGNC), may constitute a predominant group of organisms in oral sites. This study aimed to characterize phylogenetically 10 AAGNC isolated from endodontic lesions and periodontal pockets. Methods: 16S rDNA sequence and G + C content were determined. Strains sharing more than 98% sequence similarities and similar G + C content were considered the same bacterial species. Results: One isolate resembled Dialister pneumosintes (the type species of the genus Dialister) with 35 mol% G+C content and 97% sequence similarity. Of eight isolates having 45-47 mol% G+C content, seven were identified as D. invisus and one resembled Dialister invisus with 97% sequence similarity. However the 16S rDNA sequence similarities with D. pneumosintes were relatively low, indicating the strains may belong to a new genus. The last isolate revealed 35 mol% G+C content, but had higher 16S rDNA sequence similarity with D. invisus than with D. pneumosintes. Conclusion: The group of oral AAGNC isolates need to be reclassified.

Original languageEnglish
Pages (from-to)28-31
Number of pages4
JournalOral Microbiology and Immunology
Volume21
Issue number1
DOIs
Publication statusPublished - 1 Feb 2006

Keywords

  • Asaccharolytic anaerobic gram-negative anaerobic coccobacilli, AAGNC
  • Dialister invisus
  • Dialister pneumosintes
  • Gram-negative anaerobic coccobacilli
  • Phylogenetic characterization

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