TY - JOUR
T1 - Asaccharolytic anaerobic gram-negative coccobacilli (AAGNC) isolated from infected root canals and periodontal pockets
AU - Djais, A.
AU - Nakazawa, F.
AU - Sato, M.
AU - Sato, N.
AU - Sundqvist, G.
AU - Hoshino, E.
PY - 2006/2
Y1 - 2006/2
N2 - Background/aims: Culture-difficult bacteria, including asaccharolytic anaerobic gram-negative coccobacilli (AAGNC), may constitute a predominant group of organisms in oral sites. This study aimed to characterize phylogenetically 10 AAGNC isolated from endodontic lesions and periodontal pockets. Methods: 16S rDNA sequence and G + C content were determined. Strains sharing more than 98% sequence similarities and similar G + C content were considered the same bacterial species. Results: One isolate resembled Dialister pneumosintes (the type species of the genus Dialister) with 35 mol% G+C content and 97% sequence similarity. Of eight isolates having 45-47 mol% G+C content, seven were identified as D. invisus and one resembled Dialister invisus with 97% sequence similarity. However the 16S rDNA sequence similarities with D. pneumosintes were relatively low, indicating the strains may belong to a new genus. The last isolate revealed 35 mol% G+C content, but had higher 16S rDNA sequence similarity with D. invisus than with D. pneumosintes. Conclusion: The group of oral AAGNC isolates need to be reclassified.
AB - Background/aims: Culture-difficult bacteria, including asaccharolytic anaerobic gram-negative coccobacilli (AAGNC), may constitute a predominant group of organisms in oral sites. This study aimed to characterize phylogenetically 10 AAGNC isolated from endodontic lesions and periodontal pockets. Methods: 16S rDNA sequence and G + C content were determined. Strains sharing more than 98% sequence similarities and similar G + C content were considered the same bacterial species. Results: One isolate resembled Dialister pneumosintes (the type species of the genus Dialister) with 35 mol% G+C content and 97% sequence similarity. Of eight isolates having 45-47 mol% G+C content, seven were identified as D. invisus and one resembled Dialister invisus with 97% sequence similarity. However the 16S rDNA sequence similarities with D. pneumosintes were relatively low, indicating the strains may belong to a new genus. The last isolate revealed 35 mol% G+C content, but had higher 16S rDNA sequence similarity with D. invisus than with D. pneumosintes. Conclusion: The group of oral AAGNC isolates need to be reclassified.
KW - Asaccharolytic anaerobic gram-negative anaerobic coccobacilli, AAGNC
KW - Dialister invisus
KW - Dialister pneumosintes
KW - Gram-negative anaerobic coccobacilli
KW - Phylogenetic characterization
UR - http://www.scopus.com/inward/record.url?scp=33645065149&partnerID=8YFLogxK
U2 - 10.1111/j.1399-302X.2005.00249.x
DO - 10.1111/j.1399-302X.2005.00249.x
M3 - Article
C2 - 16390338
AN - SCOPUS:33645065149
SN - 0902-0055
VL - 21
SP - 28
EP - 31
JO - Oral Microbiology and Immunology
JF - Oral Microbiology and Immunology
IS - 1
ER -