TY - JOUR
T1 - ARID1A expression is down-regulated by oxidative stress in endometriosis and endometriosis-associated ovarian cancer
AU - Winarto, Hariyono
AU - Tan, Marselina Irasonia
AU - Sadikin, Mohamad
AU - Wanandi, Septelia Inawati
N1 - Publisher Copyright:
© The Author(s) 2017.
PY - 2017/2/24
Y1 - 2017/2/24
N2 - Oxidative stress is considered an important factor in the development of endometriosis, including its malignant transformation. Previous studies have found that AT-rich interactive domain 1A (ARID1A), a tumor suppressor gene, is frequently mutated and inactivated in endometriosis-associated ovarian cancer (EAOC), and such a change in this gene is considered an early event in malignant transformation. We observed oxidative stress status by measuring the activity of the antioxidant enzyme manganese superoxide dismutase (MnSOD), malondialdehyde (MDA), and ARID1A gene expression in tissue samples from patients with endometriosis, EAOC, or non–endometriosisassociated ovarian cancer (non-EAOC). We also induced oxidative stress in the cultured cells from patients with primary endometriosis by adding H2O2 and tested for any alteration of ARID1A gene expression based on different H2O2 concentrations. The results showed that MnSOD activity in endometriosis and EAOC was lower than in non-EAOC, but MDA levels were higher. This study also showed that oxidative stress reduced ARID1A gene expression.
AB - Oxidative stress is considered an important factor in the development of endometriosis, including its malignant transformation. Previous studies have found that AT-rich interactive domain 1A (ARID1A), a tumor suppressor gene, is frequently mutated and inactivated in endometriosis-associated ovarian cancer (EAOC), and such a change in this gene is considered an early event in malignant transformation. We observed oxidative stress status by measuring the activity of the antioxidant enzyme manganese superoxide dismutase (MnSOD), malondialdehyde (MDA), and ARID1A gene expression in tissue samples from patients with endometriosis, EAOC, or non–endometriosisassociated ovarian cancer (non-EAOC). We also induced oxidative stress in the cultured cells from patients with primary endometriosis by adding H2O2 and tested for any alteration of ARID1A gene expression based on different H2O2 concentrations. The results showed that MnSOD activity in endometriosis and EAOC was lower than in non-EAOC, but MDA levels were higher. This study also showed that oxidative stress reduced ARID1A gene expression.
KW - ARID1A
KW - Endometriosis
KW - Malignant transformation
KW - Ovarian cancer
KW - Oxidative stress
KW - Tumor suppressor gene
UR - http://www.scopus.com/inward/record.url?scp=85014825130&partnerID=8YFLogxK
U2 - 10.1177/1177272716689818
DO - 10.1177/1177272716689818
M3 - Article
AN - SCOPUS:85014825130
VL - 9
JO - Translational Oncogenomics
JF - Translational Oncogenomics
SN - 1177-2727
ER -