TY - JOUR
T1 - Application of ultrasound-assisted extraction on the stem bark of rhinachantus nasutus (L.) Kurz, total phenolic, and its potential as antioxidant and inhibitor of alpha-glucosidase enzyme activity
AU - Irawan, Candra
AU - Elya, Berna
AU - Hanafi, Muhammad
AU - Saputri, Fadlina Chany
N1 - Funding Information:
This work was supported by Doctoral Dissertation Research 2021 [Nomor: NKB-295/UN2.RST/HKP.05.00/] from Ministry of Research and Technology / National Research and Innovation Agency, Indonesia.
Publisher Copyright:
© 2021 Phcogj.Com.
PY - 2021/9
Y1 - 2021/9
N2 - Aims: This study aims to obtain a stem bark extract of Rhinachantus nasutus (L.) Kurz through the application of ultrasound-assisted extraction (UAE) and reveal: the total phenolic content in the extract; The extract's potential as an antioxidant with copper-reducing strength parameters, and its potential as an antidiabetic by inhibiting alpha-glucosidase activity. Results: The crude ethanol extract of R. nasutus stem bark obtained from the UAE process was 7.4896 g with a yield of 4.99%. The high total phenolic content, namely 677.3343±0.0007 mg GAE / g sample, the antioxidant activity test using the CUPRAC method gave an IC50 value of 18.43±0.20 mg / L. In addition, the ethanol extract of stem bark has a high ability to inhibit the activity of the alpha-glucosidase enzyme with an IC50 value of 10.95±0.28 mg / L. Conclusion: The ethanol extract of the stem bark of R. nasutus from UAE has the potential as a source of antioxidants and antidiabetic.
AB - Aims: This study aims to obtain a stem bark extract of Rhinachantus nasutus (L.) Kurz through the application of ultrasound-assisted extraction (UAE) and reveal: the total phenolic content in the extract; The extract's potential as an antioxidant with copper-reducing strength parameters, and its potential as an antidiabetic by inhibiting alpha-glucosidase activity. Results: The crude ethanol extract of R. nasutus stem bark obtained from the UAE process was 7.4896 g with a yield of 4.99%. The high total phenolic content, namely 677.3343±0.0007 mg GAE / g sample, the antioxidant activity test using the CUPRAC method gave an IC50 value of 18.43±0.20 mg / L. In addition, the ethanol extract of stem bark has a high ability to inhibit the activity of the alpha-glucosidase enzyme with an IC50 value of 10.95±0.28 mg / L. Conclusion: The ethanol extract of the stem bark of R. nasutus from UAE has the potential as a source of antioxidants and antidiabetic.
KW - Alpha-glucosidase enzyme
KW - Antidiabetic
KW - Antioxidant
KW - Rhinachantus nasutus (L.) Kurz
KW - Total phenolics content
KW - Ultrasound-assisted extraction
UR - http://www.scopus.com/inward/record.url?scp=85115307049&partnerID=8YFLogxK
U2 - 10.5530/pj.2021.13.164
DO - 10.5530/pj.2021.13.164
M3 - Article
AN - SCOPUS:85115307049
SN - 0975-3575
VL - 13
SP - 1297
EP - 1303
JO - Pharmacognosy Journal
JF - Pharmacognosy Journal
IS - 5
ER -