TY - JOUR
T1 - Application of Ionic Liquids based Microwave-Assisted Extraction to Bioactive Compounds Comfrey (Symphytum officinale L.) Leaves
AU - Hasan, Nahrul
AU - Mun'im, Abdul
AU - Hayun, Hayun
PY - 2022/3
Y1 - 2022/3
N2 - Extraction of bioactive compounds from comfrey leaves (Symphytum officinale L.) was carried out by comparing organic solvents and ionic liquids between conventional and microwave-assisted extraction (IL-MAE) methods. The comfrey leaf powder was extracted under various conditions. The types of organic solvents used are 65% ethanol and ionic liquids. The extraction methods used are reflux and microwave-assisted extraction. The ionic liquid was extracted by microwave-assisted extraction with the following conditions: ionic liquid concentration of 1 mol/L, the solid-liquid ratio of 1:20 (g/mL), extraction time of 10 minutes, and power of 30% (270 Watt). The total phenolic content of the extract was analyzed with a microplate reader. Allantoin and retrorsine N-oxide levels were analyzed by thin-layer chromatography – densitometry. MAE extraction in a solvent containing 65% ethanol resulted in the highest total phenolic content of 1.038±0.012. The highest levels of allantoin were also obtained from MAE extraction with 65% ethanol solvent at 2.922 ± 0.123. The highest levels of retrorsine N-oxide were obtained by extraction of MAE with (BMIM)Br with a concentration of 0.049 ± 0.007. Extraction of comfrey leaves using the MAE method of 65% ethanol produced the highest total phenolic and allantoin content compared to other methods. (BMIM) Br extraction with MAE can attract the highest retrorsine N-oxide in comfrey leaves.
AB - Extraction of bioactive compounds from comfrey leaves (Symphytum officinale L.) was carried out by comparing organic solvents and ionic liquids between conventional and microwave-assisted extraction (IL-MAE) methods. The comfrey leaf powder was extracted under various conditions. The types of organic solvents used are 65% ethanol and ionic liquids. The extraction methods used are reflux and microwave-assisted extraction. The ionic liquid was extracted by microwave-assisted extraction with the following conditions: ionic liquid concentration of 1 mol/L, the solid-liquid ratio of 1:20 (g/mL), extraction time of 10 minutes, and power of 30% (270 Watt). The total phenolic content of the extract was analyzed with a microplate reader. Allantoin and retrorsine N-oxide levels were analyzed by thin-layer chromatography – densitometry. MAE extraction in a solvent containing 65% ethanol resulted in the highest total phenolic content of 1.038±0.012. The highest levels of allantoin were also obtained from MAE extraction with 65% ethanol solvent at 2.922 ± 0.123. The highest levels of retrorsine N-oxide were obtained by extraction of MAE with (BMIM)Br with a concentration of 0.049 ± 0.007. Extraction of comfrey leaves using the MAE method of 65% ethanol produced the highest total phenolic and allantoin content compared to other methods. (BMIM) Br extraction with MAE can attract the highest retrorsine N-oxide in comfrey leaves.
KW - Symphytum officinale L.
KW - Ionic liquid
KW - Microwave-assisted extraction
KW - Bioactive compounds
UR - https://aisyah.journalpress.id/index.php/jika/article/view/7105
U2 - 10.30604/jika.v7i1.764
DO - 10.30604/jika.v7i1.764
M3 - Article
SN - 2502-4825
VL - 7
SP - 39
EP - 46
JO - Jurnal Aisyah : Jurnal Ilmu Kesehatan
JF - Jurnal Aisyah : Jurnal Ilmu Kesehatan
IS - 1
ER -