@inproceedings{bacc7a9d9fb1403499887a2ab9897bb7,
title = "Application of immunohistochemistry methods for detecting of betanodavirus in tiger grouper fish (Epinephelus fuscoguttatus)",
abstract = "Detection of betanodavirus antigen on the heads of infected tiger grouper fish (Epinephelus fuscoguttatus) by immunohistochemistry has been done. The clinical sign of the infected larva and juvenile stages often show abnormal swimming behavior, including vertical positioning, spinning and some change in pigmentation. Methods of screening done by RT-PCR positive result showed by electrophoresis band in 230 base pairs. Histopathologically, there was necrotic area with vacuolation in brain and retine organs. Immunohistochemistry detection using specific monoclonal antibody to betanodavirus showed reaction with brown colors formation in the internal organs such as brain and retine. Immunohistochemistry assay is one of the suitable methods for detecting and diagnosing of betanodavirus infection in fish.",
keywords = "Biological aspect, Demersal fish, Density, Distribution, South China Sea",
author = "S. Andriyanto and A. Abinawanto and A. Salamah",
note = "Publisher Copyright: {\textcopyright} 2018 Author(s).; 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017 ; Conference date: 26-07-2017 Through 27-07-2017",
year = "2018",
month = oct,
day = "22",
doi = "10.1063/1.5064124",
language = "English",
series = "AIP Conference Proceedings",
publisher = "American Institute of Physics Inc.",
editor = "Ratna Yuniati and Terry Mart and Anggraningrum, {Ivandini T.} and Djoko Triyono and Sugeng, {Kiki A.}",
booktitle = "Proceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017",
}