Application of immunohistochemistry methods for detecting of betanodavirus in tiger grouper fish (Epinephelus fuscoguttatus)

S. Andriyanto, Abinawanto, Andi Salamah

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Detection of betanodavirus antigen on the heads of infected tiger grouper fish (Epinephelus fuscoguttatus) by immunohistochemistry has been done. The clinical sign of the infected larva and juvenile stages often show abnormal swimming behavior, including vertical positioning, spinning and some change in pigmentation. Methods of screening done by RT-PCR positive result showed by electrophoresis band in 230 base pairs. Histopathologically, there was necrotic area with vacuolation in brain and retine organs. Immunohistochemistry detection using specific monoclonal antibody to betanodavirus showed reaction with brown colors formation in the internal organs such as brain and retine. Immunohistochemistry assay is one of the suitable methods for detecting and diagnosing of betanodavirus infection in fish.

Original languageEnglish
Title of host publicationProceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
EditorsRatna Yuniati, Terry Mart, Ivandini T. Anggraningrum, Djoko Triyono, Kiki A. Sugeng
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735417410
DOIs
Publication statusPublished - 22 Oct 2018
Event3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017 - Bali, Indonesia
Duration: 26 Jul 201727 Jul 2017

Publication series

NameAIP Conference Proceedings
Volume2023
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
CountryIndonesia
CityBali
Period26/07/1727/07/17

Keywords

  • Biological aspect
  • Demersal fish
  • Density
  • Distribution
  • South China Sea

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