Application of human adipose stem cell (hADSC) in collagen gel on rat deep dermal burn

Helsy Junaidi; Puji Sari; Dwi Pratami Septiara; Jeanne A. Pawitan; Luluk Yunaini

doi:10.3844/ojbsci.2019.192.202

Application of human adipose stem cell (hADSC) in collagen gel on rat deep dermal burn

Helsy Junaidi, Puji Sari, Dwi Pratami Septiara, Jeanne A. Pawitan, Luluk Yunaini

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Human adipose derived stem cells (hADSC) is expected to be an efficient solution for healing deep-dermal burns that help in wound closure. This study used 20 male Sprague dawley rats. Each rat received three wounds with different treatments (control, hADSC in collagen gel and collagen gel). Wound closure was observed at day 7th, 14th, 21th and 28th (sacrificed day) macroscopically, wound areas were measured and collagen densities were evaluated. Reduction of wound area showed significant difference between hADSC in collagen gel group and control group (p 0.001) and between hADSC in collagen gel group and collagen gel group. The hADSC in collagen gel group had a higher collagen density compared to control and collagen gel group. Application of hADSC in bovine collagen gel in rat deep-dermal burn models provided faster and better wound healing compared with control and bovine collagen gel group.

Original language	English
Pages (from-to)	192-202
Number of pages	11
Journal	OnLine Journal of Biological Sciences
Volume	19
Issue number	3
DOIs	https://doi.org/10.3844/ojbsci.2019.192.202
Publication status	Published - 1 Jan 2019

Keywords

Bovine Collagen Gel
Burns
HADSC
PCR

Access to Document

10.3844/ojbsci.2019.192.202

Cite this

@article{d7f2fde211aa436c94568af569e2dadb,

title = "Application of human adipose stem cell (hADSC) in collagen gel on rat deep dermal burn",

abstract = "Human adipose derived stem cells (hADSC) is expected to be an efficient solution for healing deep-dermal burns that help in wound closure. This study used 20 male Sprague dawley rats. Each rat received three wounds with different treatments (control, hADSC in collagen gel and collagen gel). Wound closure was observed at day 7th, 14th, 21th and 28th (sacrificed day) macroscopically, wound areas were measured and collagen densities were evaluated. Reduction of wound area showed significant difference between hADSC in collagen gel group and control group (p 0.001) and between hADSC in collagen gel group and collagen gel group. The hADSC in collagen gel group had a higher collagen density compared to control and collagen gel group. Application of hADSC in bovine collagen gel in rat deep-dermal burn models provided faster and better wound healing compared with control and bovine collagen gel group.",

keywords = "Bovine Collagen Gel, Burns, HADSC, PCR",

author = "Helsy Junaidi and Puji Sari and Septiara, {Dwi Pratami} and Pawitan, {Jeanne A.} and Luluk Yunaini",

note = "Publisher Copyright: {\textcopyright} 2019 Helsy Junaidi, Puji Sari, Dwi Pratami Septiara, Jeanne A. Pawitan and Luluk Yunaini.",

year = "2019",

month = jan,

day = "1",

doi = "10.3844/ojbsci.2019.192.202",

language = "English",

volume = "19",

pages = "192--202",

journal = "OnLine Journal of Biological Sciences",

issn = "1608-4217",

publisher = "Science Publications",

number = "3",

}

TY - JOUR

T1 - Application of human adipose stem cell (hADSC) in collagen gel on rat deep dermal burn

AU - Junaidi, Helsy

AU - Sari, Puji

AU - Septiara, Dwi Pratami

AU - Pawitan, Jeanne A.

AU - Yunaini, Luluk

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Human adipose derived stem cells (hADSC) is expected to be an efficient solution for healing deep-dermal burns that help in wound closure. This study used 20 male Sprague dawley rats. Each rat received three wounds with different treatments (control, hADSC in collagen gel and collagen gel). Wound closure was observed at day 7th, 14th, 21th and 28th (sacrificed day) macroscopically, wound areas were measured and collagen densities were evaluated. Reduction of wound area showed significant difference between hADSC in collagen gel group and control group (p 0.001) and between hADSC in collagen gel group and collagen gel group. The hADSC in collagen gel group had a higher collagen density compared to control and collagen gel group. Application of hADSC in bovine collagen gel in rat deep-dermal burn models provided faster and better wound healing compared with control and bovine collagen gel group.

AB - Human adipose derived stem cells (hADSC) is expected to be an efficient solution for healing deep-dermal burns that help in wound closure. This study used 20 male Sprague dawley rats. Each rat received three wounds with different treatments (control, hADSC in collagen gel and collagen gel). Wound closure was observed at day 7th, 14th, 21th and 28th (sacrificed day) macroscopically, wound areas were measured and collagen densities were evaluated. Reduction of wound area showed significant difference between hADSC in collagen gel group and control group (p 0.001) and between hADSC in collagen gel group and collagen gel group. The hADSC in collagen gel group had a higher collagen density compared to control and collagen gel group. Application of hADSC in bovine collagen gel in rat deep-dermal burn models provided faster and better wound healing compared with control and bovine collagen gel group.

KW - Bovine Collagen Gel

KW - Burns

KW - HADSC

KW - PCR

UR - http://www.scopus.com/inward/record.url?scp=85074078234&partnerID=8YFLogxK

U2 - 10.3844/ojbsci.2019.192.202

DO - 10.3844/ojbsci.2019.192.202

M3 - Article

AN - SCOPUS:85074078234

SN - 1608-4217

VL - 19

SP - 192

EP - 202

JO - OnLine Journal of Biological Sciences

JF - OnLine Journal of Biological Sciences

IS - 3

ER -

Application of human adipose stem cell (hADSC) in collagen gel on rat deep dermal burn

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this