Application of homogenization process to the hydrolysis technique on removing mucilaginous sheath of branched filamentous Cyanobacteria

K. D.P. Loka, D. Hendrayanti, A. A. Nasrul, S. W.R. Hilia, R. Lestari

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Research on the optimization of hydrolysis techniques on the removal of mucilaginous sheath branched filamentous Cyanobacteria has been carried out. The optimizations included the homogenizing process and addition of nitrogen in growth medium. The aim of the study is to analyze the process of hydrolysis based on the absence and presence of Nitrogen in culture medium and to analyze the effect of hydrolysis on removing the mucilaginous sheath. The hydrolysis treatment was performed on three strains of branched filamentous Cyanobacteria were used in this study: SO-130, SO-133 and SO-198 using five replications with homogenization process used five glass beads BioSpect 3.2 mm. The results were observed using microscopic observations with and without negative staining. The result showed that the mucilaginous sheath of three strains was disappeared after hydrolysis process that growth in medium with and without nitrogen. The DNA purity in all treatments was 0.81-1.19, indicating the absence of pure DNA. The DNA concentration were 1.63-6.47 ng/μL and have the same pattern in the three strains that was decreasing from control to treatment.

Original languageEnglish
Title of host publicationProceedings of the 4th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2018
EditorsTerry Mart, Djoko Triyono, Ivandini T. Anggraningrum
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735419155
DOIs
Publication statusPublished - 4 Nov 2019
Event4th International Symposium on Current Progress in Mathematics and Sciences 2018, ISCPMS 2018 - Depok, Indonesia
Duration: 30 Oct 201831 Oct 2018

Publication series

NameAIP Conference Proceedings
Volume2168
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference4th International Symposium on Current Progress in Mathematics and Sciences 2018, ISCPMS 2018
CountryIndonesia
CityDepok
Period30/10/1831/10/18

Keywords

  • Glass beads
  • mucilaginous sheath
  • SO-130 strain
  • SO-133 strain
  • SO-198 strain

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