TY - JOUR
T1 - Application of a sepsis flow chip (SFC) assay for the molecular diagnosis of paediatric sepsis
AU - Prasetyo, Dimas seto
AU - Karyanti, Mulya rahma
AU - Yuniar, Irene
AU - Saharman, Yulia rosa
AU - Holiwono, Livya
PY - 2023/4/1
Y1 - 2023/4/1
N2 - A delay in detecting sepsis pathogens is a problematic issue for determining definitive antibiotic therapy for the causative pathogens. The gold standard method for sepsis is blood culture but this requires 3 days to detect the definitive pathogen. Molecular methods offer rapid identification of pathogens. We evaluated the use of sepsis flow chip (SFC) assay for identifying pathogens from children with sepsis. Blood samples from children with sepsis were collected and incubated in a culture device. Positive samples were subjected to amplification-hybridization using SFC assay and culture. A total of 94 samples from 47 patients were recovered, from which 25 isolates were recovered, including Klebsiella pneumoniae (11) and Staphylococcus epidermidis (6). From 25 positive blood culture bottles subjected to SFC assay, 24 genus/species and 18 resistance genes were detected. The sensitivity, specificity and conformity was 80, 94.2 and 94.68 % respectively. SFC assay offers promise to identify pathogens from positive blood culture in paediatric patients with sepsis and may support the antimicrobial stewardship programme in hospitals.
AB - A delay in detecting sepsis pathogens is a problematic issue for determining definitive antibiotic therapy for the causative pathogens. The gold standard method for sepsis is blood culture but this requires 3 days to detect the definitive pathogen. Molecular methods offer rapid identification of pathogens. We evaluated the use of sepsis flow chip (SFC) assay for identifying pathogens from children with sepsis. Blood samples from children with sepsis were collected and incubated in a culture device. Positive samples were subjected to amplification-hybridization using SFC assay and culture. A total of 94 samples from 47 patients were recovered, from which 25 isolates were recovered, including Klebsiella pneumoniae (11) and Staphylococcus epidermidis (6). From 25 positive blood culture bottles subjected to SFC assay, 24 genus/species and 18 resistance genes were detected. The sensitivity, specificity and conformity was 80, 94.2 and 94.68 % respectively. SFC assay offers promise to identify pathogens from positive blood culture in paediatric patients with sepsis and may support the antimicrobial stewardship programme in hospitals.
KW - amplification-hybridization
KW - paediatric sepsis
KW - blood culture
KW - antimicrobial stewardship
UR - https://www.microbiologyresearch.org/content/journal/acmi/10.1099/acmi.0.000474.v4
U2 - 10.1099/acmi.0.000474.v4
DO - 10.1099/acmi.0.000474.v4
M3 - Article
SN - 2516-8290
VL - 5
JO - Access Microbiology
JF - Access Microbiology
IS - 4
ER -