Apoptotic activity of alpha-mangostin in acetaldehyde- induced LX2 hepatic stellate cell lines

Novriantika Lestari, Daniel Amartya, Vivian Soetikno, Melva Louisa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Previous studies have shown that alpha-mangostin has cytotoxic potentials by increasing apoptotic and promoting arrest in the cell cycle process in hepatocellular carcinoma in vitro. Prior research has also shown that alpha- mangostin ameliorates alcohol's toxicity in hepatic stellate cells in vitro; however, whether alpha-mangostin exerts its effect by inducing apoptosis is still unknown. The study was aimed to analyze the apoptotic properties of alpha-mangostin in acetaldehyde-induced hepatic stellate cells in vitro. Immortalized human hepatic stellate (HSC) cells with LX2 cell line were treated with acetaldehyde in the presence or absence of alpha-mangostin (10 and 20 μM). After treatments, cells were harvested, isolated for RNA, and analyzed for mRNA expressions of apoptotic markers: Bax, Bcl-2, caspase-3, and caspase-9 using qRT-PCR. Acetaldehyde treatment caused a significant reduction in the mRNA expressions of Bax and caspase-3 and a slight decrease in the expressions of caspase-9. The addition of alpha-mangostin increased the mRNA expressions of Bax, caspase-9, and caspase-3 significantly. Increased mRNA expressions of Bcl-2 in alpha-mangostin- treated cells were observed. These effects displayed a dose-dependent tendency. Alpha-mangostin demonstrates apoptotic activity in acetaldehyde-induce hepatic stellate cells in a dose-dependent manner.

Original languageEnglish
Title of host publication4th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices
Subtitle of host publicationProceedings of the International Symposium of Biomedical Engineering, ISBE 2019
EditorsKenny Lischer, Tomy Abuzairi, Siti Fauziyah Rahman, Misri Gozan
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735419445
DOIs
Publication statusPublished - 10 Dec 2019
Event4th International Symposium of Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices, ISBE 2019 - Padang, West Sumatera, Indonesia
Duration: 22 Jul 201924 Jul 2019

Publication series

NameAIP Conference Proceedings
Volume2193
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference4th International Symposium of Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices, ISBE 2019
CountryIndonesia
CityPadang, West Sumatera
Period22/07/1924/07/19

Keywords

  • Acetaldehyde
  • alpha-mangostin
  • apoptosis
  • hepatic stellate cells

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    Lestari, N., Amartya, D., Soetikno, V., & Louisa, M. (2019). Apoptotic activity of alpha-mangostin in acetaldehyde- induced LX2 hepatic stellate cell lines. In K. Lischer, T. Abuzairi, S. F. Rahman, & M. Gozan (Eds.), 4th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2019 [030019] (AIP Conference Proceedings; Vol. 2193). American Institute of Physics Inc.. https://doi.org/10.1063/1.5139356