TY - GEN
T1 - Apoptotic activity of alpha-mangostin in acetaldehyde- induced LX2 hepatic stellate cell lines
AU - Lestari, Novriantika
AU - Amartya, Daniel
AU - Soetikno, Vivian
AU - Louisa, Melva
N1 - Publisher Copyright:
© 2019 Author(s).
PY - 2019/12/10
Y1 - 2019/12/10
N2 - Previous studies have shown that alpha-mangostin has cytotoxic potentials by increasing apoptotic and promoting arrest in the cell cycle process in hepatocellular carcinoma in vitro. Prior research has also shown that alpha- mangostin ameliorates alcohol's toxicity in hepatic stellate cells in vitro; however, whether alpha-mangostin exerts its effect by inducing apoptosis is still unknown. The study was aimed to analyze the apoptotic properties of alpha-mangostin in acetaldehyde-induced hepatic stellate cells in vitro. Immortalized human hepatic stellate (HSC) cells with LX2 cell line were treated with acetaldehyde in the presence or absence of alpha-mangostin (10 and 20 μM). After treatments, cells were harvested, isolated for RNA, and analyzed for mRNA expressions of apoptotic markers: Bax, Bcl-2, caspase-3, and caspase-9 using qRT-PCR. Acetaldehyde treatment caused a significant reduction in the mRNA expressions of Bax and caspase-3 and a slight decrease in the expressions of caspase-9. The addition of alpha-mangostin increased the mRNA expressions of Bax, caspase-9, and caspase-3 significantly. Increased mRNA expressions of Bcl-2 in alpha-mangostin- treated cells were observed. These effects displayed a dose-dependent tendency. Alpha-mangostin demonstrates apoptotic activity in acetaldehyde-induce hepatic stellate cells in a dose-dependent manner.
AB - Previous studies have shown that alpha-mangostin has cytotoxic potentials by increasing apoptotic and promoting arrest in the cell cycle process in hepatocellular carcinoma in vitro. Prior research has also shown that alpha- mangostin ameliorates alcohol's toxicity in hepatic stellate cells in vitro; however, whether alpha-mangostin exerts its effect by inducing apoptosis is still unknown. The study was aimed to analyze the apoptotic properties of alpha-mangostin in acetaldehyde-induced hepatic stellate cells in vitro. Immortalized human hepatic stellate (HSC) cells with LX2 cell line were treated with acetaldehyde in the presence or absence of alpha-mangostin (10 and 20 μM). After treatments, cells were harvested, isolated for RNA, and analyzed for mRNA expressions of apoptotic markers: Bax, Bcl-2, caspase-3, and caspase-9 using qRT-PCR. Acetaldehyde treatment caused a significant reduction in the mRNA expressions of Bax and caspase-3 and a slight decrease in the expressions of caspase-9. The addition of alpha-mangostin increased the mRNA expressions of Bax, caspase-9, and caspase-3 significantly. Increased mRNA expressions of Bcl-2 in alpha-mangostin- treated cells were observed. These effects displayed a dose-dependent tendency. Alpha-mangostin demonstrates apoptotic activity in acetaldehyde-induce hepatic stellate cells in a dose-dependent manner.
KW - Acetaldehyde
KW - alpha-mangostin
KW - apoptosis
KW - hepatic stellate cells
UR - http://www.scopus.com/inward/record.url?scp=85076759608&partnerID=8YFLogxK
U2 - 10.1063/1.5139356
DO - 10.1063/1.5139356
M3 - Conference contribution
AN - SCOPUS:85076759608
T3 - AIP Conference Proceedings
BT - 4th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices
A2 - Lischer, Kenny
A2 - Abuzairi, Tomy
A2 - Rahman, Siti Fauziyah
A2 - Gozan, Misri
PB - American Institute of Physics Inc.
T2 - 4th International Symposium of Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices, ISBE 2019
Y2 - 22 July 2019 through 24 July 2019
ER -