Apoptin gene optimization in Escherichia coli

Muhamad Sahlan; Ihsan Wiratama; Heri Hermansyah; Anondho Wijarnako; Mohamad Teguh Gumelar; Masafumi Yohda

doi:10.1063/1.5096733

Apoptin gene optimization in Escherichia coli

Muhamad Sahlan
, Ihsan Wiratama
, Heri Hermansyah
, Anondho Wijarnako
, Mohamad Teguh Gumelar
, Masafumi Yohda

Department of Chemical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Apoptin is intensively studied for its potential as a therapeutic protein for cancer treatment since it is capable of inducing apoptosis specifically in cancer cells. Production of apoptin gene on a larger scale is important for the further application, and currently being studied by researchers around the world. In this research, chicken anemia virus apoptin optimized genetically for expression in Escherichia coli and modified using (His)6 tag, (Arg)8 tag, and HlyA tag intended for purification needs, penetration enhancement, and secretion from bacterial host to the growth media. Removal of (His)6 tag and HlyA tag were designed using a specific thrombin proteolytic site, while (Arg)8 tag was preserved. The gene designed, optimized and constructed in vector pET9a, then analyzed using Agarose Electrophoresis and Sequencing. The DNA sequencing result shows that the designed gene has successfully constructed.

Original language	English
Title of host publication	3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
Subtitle of host publication	Proceedings of the International Symposium of Biomedical Engineering, ISBE 2018
Editors	Praswasti P.D.K. Wulan, Misri Gozan, Sotya Astutiningsih, Ghiska Ramahdita, Radon Dhelika, Prasetyanugraheni Kreshanti
Publisher	American Institute of Physics Inc.
ISBN (Electronic)	9780735418226
DOIs	https://doi.org/10.1063/1.5096733
Publication status	Published - 9 Apr 2019
Event	3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018 - Jakarta, Indonesia Duration: 6 Aug 2018 → 8 Aug 2018

Publication series

Name	AIP Conference Proceedings
Volume	2092
ISSN (Print)	0094-243X
ISSN (Electronic)	1551-7616

Conference

Conference	3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018
Country/Territory	Indonesia
City	Jakarta
Period	6/08/18 → 8/08/18

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

apoptin
codon optimization

Access to Document

10.1063/1.5096733

Cite this

Sahlan, M., Wiratama, I., Hermansyah, H., Wijarnako, A., Gumelar, M. T., & Yohda, M. (2019). Apoptin gene optimization in Escherichia coli. In P. P. D. K. Wulan, M. Gozan, S. Astutiningsih, G. Ramahdita, R. Dhelika, & P. Kreshanti (Eds.), 3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2018 Article 030029 (AIP Conference Proceedings; Vol. 2092). American Institute of Physics Inc.. https://doi.org/10.1063/1.5096733

Sahlan, Muhamad ; Wiratama, Ihsan ; Hermansyah, Heri et al. / Apoptin gene optimization in Escherichia coli. 3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2018. editor / Praswasti P.D.K. Wulan ; Misri Gozan ; Sotya Astutiningsih ; Ghiska Ramahdita ; Radon Dhelika ; Prasetyanugraheni Kreshanti. American Institute of Physics Inc., 2019. (AIP Conference Proceedings).

@inproceedings{079ff9150c764a4a8aebed0b81a67666,

title = "Apoptin gene optimization in Escherichia coli",

abstract = "Apoptin is intensively studied for its potential as a therapeutic protein for cancer treatment since it is capable of inducing apoptosis specifically in cancer cells. Production of apoptin gene on a larger scale is important for the further application, and currently being studied by researchers around the world. In this research, chicken anemia virus apoptin optimized genetically for expression in Escherichia coli and modified using (His)6 tag, (Arg)8 tag, and HlyA tag intended for purification needs, penetration enhancement, and secretion from bacterial host to the growth media. Removal of (His)6 tag and HlyA tag were designed using a specific thrombin proteolytic site, while (Arg)8 tag was preserved. The gene designed, optimized and constructed in vector pET9a, then analyzed using Agarose Electrophoresis and Sequencing. The DNA sequencing result shows that the designed gene has successfully constructed.",

keywords = "apoptin, codon optimization",

author = "Muhamad Sahlan and Ihsan Wiratama and Heri Hermansyah and Anondho Wijarnako and Gumelar, \{Mohamad Teguh\} and Masafumi Yohda",

note = "Publisher Copyright: {\textcopyright} 2019 Author(s).; 3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018 ; Conference date: 06-08-2018 Through 08-08-2018",

year = "2019",

month = apr,

day = "9",

doi = "10.1063/1.5096733",

language = "English",

series = "AIP Conference Proceedings",

publisher = "American Institute of Physics Inc.",

editor = "Wulan, \{Praswasti P.D.K.\} and Misri Gozan and Sotya Astutiningsih and Ghiska Ramahdita and Radon Dhelika and Prasetyanugraheni Kreshanti",

booktitle = "3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices",

address = "United States",

}

Sahlan, M, Wiratama, I, Hermansyah, H, Wijarnako, A, Gumelar, MT & Yohda, M 2019, Apoptin gene optimization in Escherichia coli. in PPDK Wulan, M Gozan, S Astutiningsih, G Ramahdita, R Dhelika & P Kreshanti (eds), 3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2018., 030029, AIP Conference Proceedings, vol. 2092, American Institute of Physics Inc., 3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018, Jakarta, Indonesia, 6/08/18. https://doi.org/10.1063/1.5096733

Apoptin gene optimization in Escherichia coli. / Sahlan, Muhamad; Wiratama, Ihsan; Hermansyah, Heri et al.
3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2018. ed. / Praswasti P.D.K. Wulan; Misri Gozan; Sotya Astutiningsih; Ghiska Ramahdita; Radon Dhelika; Prasetyanugraheni Kreshanti. American Institute of Physics Inc., 2019. 030029 (AIP Conference Proceedings; Vol. 2092).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Apoptin gene optimization in Escherichia coli

AU - Sahlan, Muhamad

AU - Wiratama, Ihsan

AU - Hermansyah, Heri

AU - Wijarnako, Anondho

AU - Gumelar, Mohamad Teguh

AU - Yohda, Masafumi

PY - 2019/4/9

Y1 - 2019/4/9

N2 - Apoptin is intensively studied for its potential as a therapeutic protein for cancer treatment since it is capable of inducing apoptosis specifically in cancer cells. Production of apoptin gene on a larger scale is important for the further application, and currently being studied by researchers around the world. In this research, chicken anemia virus apoptin optimized genetically for expression in Escherichia coli and modified using (His)6 tag, (Arg)8 tag, and HlyA tag intended for purification needs, penetration enhancement, and secretion from bacterial host to the growth media. Removal of (His)6 tag and HlyA tag were designed using a specific thrombin proteolytic site, while (Arg)8 tag was preserved. The gene designed, optimized and constructed in vector pET9a, then analyzed using Agarose Electrophoresis and Sequencing. The DNA sequencing result shows that the designed gene has successfully constructed.

AB - Apoptin is intensively studied for its potential as a therapeutic protein for cancer treatment since it is capable of inducing apoptosis specifically in cancer cells. Production of apoptin gene on a larger scale is important for the further application, and currently being studied by researchers around the world. In this research, chicken anemia virus apoptin optimized genetically for expression in Escherichia coli and modified using (His)6 tag, (Arg)8 tag, and HlyA tag intended for purification needs, penetration enhancement, and secretion from bacterial host to the growth media. Removal of (His)6 tag and HlyA tag were designed using a specific thrombin proteolytic site, while (Arg)8 tag was preserved. The gene designed, optimized and constructed in vector pET9a, then analyzed using Agarose Electrophoresis and Sequencing. The DNA sequencing result shows that the designed gene has successfully constructed.

KW - apoptin

KW - codon optimization

UR - https://www.scopus.com/pages/publications/85064820028

U2 - 10.1063/1.5096733

DO - 10.1063/1.5096733

M3 - Conference contribution

AN - SCOPUS:85064820028

T3 - AIP Conference Proceedings

BT - 3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices

A2 - Wulan, Praswasti P.D.K.

A2 - Gozan, Misri

A2 - Astutiningsih, Sotya

A2 - Ramahdita, Ghiska

A2 - Dhelika, Radon

A2 - Kreshanti, Prasetyanugraheni

PB - American Institute of Physics Inc.

T2 - 3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018

Y2 - 6 August 2018 through 8 August 2018

ER -

Sahlan M, Wiratama I, Hermansyah H, Wijarnako A, Gumelar MT, Yohda M. Apoptin gene optimization in Escherichia coli. In Wulan PPDK, Gozan M, Astutiningsih S, Ramahdita G, Dhelika R, Kreshanti P, editors, 3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2018. American Institute of Physics Inc. 2019. 030029. (AIP Conference Proceedings). doi: 10.1063/1.5096733

Apoptin gene optimization in Escherichia coli

Abstract

Publication series

Conference

UN SDGs

Keywords

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