Apoptin gene optimization in Escherichia coli

Muhamad Sahlan, Ihsan Wiratama, Heri Hermansyah, Anondho Wijarnako, Mohamad Teguh Gumelar, Masafumi Yohda

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Apoptin is intensively studied for its potential as a therapeutic protein for cancer treatment since it is capable of inducing apoptosis specifically in cancer cells. Production of apoptin gene on a larger scale is important for the further application, and currently being studied by researchers around the world. In this research, chicken anemia virus apoptin optimized genetically for expression in Escherichia coli and modified using (His)6 tag, (Arg)8 tag, and HlyA tag intended for purification needs, penetration enhancement, and secretion from bacterial host to the growth media. Removal of (His)6 tag and HlyA tag were designed using a specific thrombin proteolytic site, while (Arg)8 tag was preserved. The gene designed, optimized and constructed in vector pET9a, then analyzed using Agarose Electrophoresis and Sequencing. The DNA sequencing result shows that the designed gene has successfully constructed.

Original languageEnglish
Title of host publication3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
Subtitle of host publicationProceedings of the International Symposium of Biomedical Engineering, ISBE 2018
EditorsPraswasti P.D.K. Wulan, Misri Gozan, Sotya Astutiningsih, Ghiska Ramahdita, Radon Dhelika, Prasetyanugraheni Kreshanti
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735418226
DOIs
Publication statusPublished - 9 Apr 2019
Event3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018 - Jakarta, Indonesia
Duration: 6 Aug 20188 Aug 2018

Publication series

NameAIP Conference Proceedings
Volume2092
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018
CountryIndonesia
CityJakarta
Period6/08/188/08/18

Keywords

  • apoptin
  • codon optimization

Fingerprint Dive into the research topics of 'Apoptin gene optimization in Escherichia coli'. Together they form a unique fingerprint.

Cite this