TY - JOUR
T1 - Antiviral Effect of Pterocarpus indicus Wild Leaves Extract Against Replication of Dengue Virus (DENV) In Vitro
AU - Dewi, Beti Ernawati
AU - Angelina, Marissa
AU - Meilawati, Lia
AU - Hartati, Sri
AU - Dewijanti, Indah Dwiatmi
AU - Santi, Mei Ria
AU - Desti, Hidayati
AU - Sudiro, Tjahjani Mirawati
PY - 2018/1
Y1 - 2018/1
N2 - Dengue hemorrhagic fever (DHF) is major public health problem in tropical and subtropical areas of the world with
lack of approved vaccines and effective antiviral therapies. With no current treatment for illness attributed to dengue
virus (DENV) infection other than supportive care, therapeutic strategies that use natural extract was developed.
Indonesia have many plants that potential for antiviral drμgs such as Pterocarpus indicus Willd (P. indicus). The
objective of this study was to determine the effect of P. indicus to inhibit DENV replication. We used a well-
differentiated hepatocytes-derived cellular carcinoma cell line (Huh-7 it-1 cells) to determine and select antiviral
activity. The toxicity effects were determined by MTT assay. Then, the suppression of DENV replication was
determined by Focus assay. Dengue infected cells with DMSO were used as control. We found that crude extract
(Pi), hexane (Pi.1) and ethyl acetate (Pi.2) extract showed strong inhibition with high selectivity index (SI) of 1,392;
285.36 and 168.56 respectively. Sub fraction of Pi.1 and Pi.2 still showed strong inhibition with high SI. Further
sub-sub fraction of Pi.2 such as Pi.2.12 and Pi.2.12.1 still showed inhibition of DENV replication but there was
reduction of SI value. The mechanism experiment of Pi.2.12, we found that Pi 2.12 more profound to inhibit in the
post infection stage that entry or pre-infection. We conclude that the sub-fraction of Pi.2.12 has potential antiviral
activity against DV infection in vitro. Further studies are still needed to investigate the pure compound of Pi.2.12
that inhibit and have advantages in the future as alternative for treatment of DENV infection.
AB - Dengue hemorrhagic fever (DHF) is major public health problem in tropical and subtropical areas of the world with
lack of approved vaccines and effective antiviral therapies. With no current treatment for illness attributed to dengue
virus (DENV) infection other than supportive care, therapeutic strategies that use natural extract was developed.
Indonesia have many plants that potential for antiviral drμgs such as Pterocarpus indicus Willd (P. indicus). The
objective of this study was to determine the effect of P. indicus to inhibit DENV replication. We used a well-
differentiated hepatocytes-derived cellular carcinoma cell line (Huh-7 it-1 cells) to determine and select antiviral
activity. The toxicity effects were determined by MTT assay. Then, the suppression of DENV replication was
determined by Focus assay. Dengue infected cells with DMSO were used as control. We found that crude extract
(Pi), hexane (Pi.1) and ethyl acetate (Pi.2) extract showed strong inhibition with high selectivity index (SI) of 1,392;
285.36 and 168.56 respectively. Sub fraction of Pi.1 and Pi.2 still showed strong inhibition with high SI. Further
sub-sub fraction of Pi.2 such as Pi.2.12 and Pi.2.12.1 still showed inhibition of DENV replication but there was
reduction of SI value. The mechanism experiment of Pi.2.12, we found that Pi 2.12 more profound to inhibit in the
post infection stage that entry or pre-infection. We conclude that the sub-fraction of Pi.2.12 has potential antiviral
activity against DV infection in vitro. Further studies are still needed to investigate the pure compound of Pi.2.12
that inhibit and have advantages in the future as alternative for treatment of DENV infection.
U2 - http://www.jtrolis.ub.ac.id/index.php/jtrolis/article/view/530/pdf
DO - http://www.jtrolis.ub.ac.id/index.php/jtrolis/article/view/530/pdf
M3 - Article
VL - 8
SP - 55
EP - 61
JO - The Journal of Tropical Life Science
JF - The Journal of Tropical Life Science
IS - 1
ER -
