TY - JOUR
T1 - Antiviral Activity of Isobutyl Gallate to Dengue Virus Serotype 2 in Vitro
AU - Dewi, B. E.
AU - Angelina, M.
AU - Nuwwaaridya, F.
AU - Desti, H.
AU - Sudiro, T. M.
N1 - Publisher Copyright:
© Published under licence by IOP Publishing Ltd.
PY - 2019/4/12
Y1 - 2019/4/12
N2 -
Dengue is one of the most prevalent mosquito-borne diseases in Indonesia that lead to a spectrum of disease, ranging from a flu-like illness to a life-threatening dengue shock syndrome. Despite its high incidence and mortality rate, there is currently no antiviral drug available against dengue virus (DENV). Therefore, research regarding the development of anti-viral to DENV is essential. The aim of this study was to evaluate the antiviral activity of isobutyl gallate, a gallic acid derivative, against DENV in vitro. Various concentration of isobutyl gallate was added to DENV-2 strain New Guinea C (NGC) and infected to Vero cell line. Inhibition of DENV replication as aligned with half inhibitory concentration (IC
50
) value was determined by focus assay. Furthermore, the toxicity of isobutyl gallate that corresponds with cytotoxic concentration (CC
50
) value was determined by MTT assay. Selectivity index (SI) value was defined as the ratio of CC
50
to IC
50
. Isobutyl gallate showed no cytotoxic effects against Huh 7 it-1 cells with CC
50
values of 167.19 μg/mL and exhibited strong antiviral activity with IC
50
of 4.45 μg/mL, hence a significant SI value of 25.69. Isobutyl gallate exerts potent antiviral activity against DENV.
AB -
Dengue is one of the most prevalent mosquito-borne diseases in Indonesia that lead to a spectrum of disease, ranging from a flu-like illness to a life-threatening dengue shock syndrome. Despite its high incidence and mortality rate, there is currently no antiviral drug available against dengue virus (DENV). Therefore, research regarding the development of anti-viral to DENV is essential. The aim of this study was to evaluate the antiviral activity of isobutyl gallate, a gallic acid derivative, against DENV in vitro. Various concentration of isobutyl gallate was added to DENV-2 strain New Guinea C (NGC) and infected to Vero cell line. Inhibition of DENV replication as aligned with half inhibitory concentration (IC
50
) value was determined by focus assay. Furthermore, the toxicity of isobutyl gallate that corresponds with cytotoxic concentration (CC
50
) value was determined by MTT assay. Selectivity index (SI) value was defined as the ratio of CC
50
to IC
50
. Isobutyl gallate showed no cytotoxic effects against Huh 7 it-1 cells with CC
50
values of 167.19 μg/mL and exhibited strong antiviral activity with IC
50
of 4.45 μg/mL, hence a significant SI value of 25.69. Isobutyl gallate exerts potent antiviral activity against DENV.
UR - http://www.scopus.com/inward/record.url?scp=85064863809&partnerID=8YFLogxK
U2 - 10.1088/1755-1315/251/1/012018
DO - 10.1088/1755-1315/251/1/012018
M3 - Conference article
AN - SCOPUS:85064863809
SN - 1755-1307
VL - 251
JO - IOP Conference Series: Earth and Environmental Science
JF - IOP Conference Series: Earth and Environmental Science
IS - 1
M1 - 012018
T2 - 2nd International Conference on Natural Products and Bioresource Sciences 2018, ICONPROBIOS 2018
Y2 - 1 November 2018 through 2 November 2018
ER -
