Antiviral activity of Ceiba pentandra and Eugenia uniflora leaf extracts to dengue virus serotype-2 in Huh 7it-1 cell line

Beti Ernawati Dewi; Marissa Angelina; Sabda Ardiantara; Agung Riyanto Prakoso; Hidayati Desti; Tjahjani Mirawati Sudiro

doi:10.1063/1.5139340

Antiviral activity of Ceiba pentandra and Eugenia uniflora leaf extracts to dengue virus serotype-2 in Huh 7it-1 cell line

Beti Ernawati Dewi, Marissa Angelina, Sabda Ardiantara, Agung Riyanto Prakoso, Hidayati Desti, Tjahjani Mirawati Sudiro

Department of Microbiology Clinic

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

5 Citations (Scopus)

Abstract

Dengue Fever (DF) is an infectious disease caused by dengue virus (DENV) infection which is transmitted by Aedes aegypti and Aedes albopictus mosquito bite. There are approximately 50 million DENV cases annually in the world with 500 thousand cases hospitalized. Incidence rate of DENV infection is increasing in Indonesia every year. Specific antiviral that can inhibit or mitigate the progression of the disease have been investigated for many decades. However, until now, management of DENV patients is only supportive. Specific antiviral drug to DENV is not available yet. The objective of this study is to determine antiviral activity of Ceiba pentandra and Eugenia uniflora leaf extract against DENV replication in Huh7it-1 cell line. Study design was an experimental study with a Focus assay and MTT assay to determine the effectivity and toxicity, respectively. Ceiba pentandra leaf extract showed IC₅₀, CC₅₀ and SI of 15.49 μg/ml, 81.1 μg/ml and 5.23. In other hand, E. uniflora revealed IC₅₀, CC₅₀, and SI of 19.83 μg/ml, 134.42 μg/ml and 6.78. We concluded that C. pentandra and E. uniflora leaf extract have potential as antiviral drug to DENV. Further studies are needed to explore the substance of C. pentandra and E. uniflora leaf extract that have antiviral activities to DENV.

Original language	English
Title of host publication	4th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices
Subtitle of host publication	Proceedings of the International Symposium of Biomedical Engineering, ISBE 2019
Editors	Kenny Lischer, Tomy Abuzairi, Siti Fauziyah Rahman, Misri Gozan
Publisher	American Institute of Physics Inc.
ISBN (Electronic)	9780735419445
DOIs	https://doi.org/10.1063/1.5139340
Publication status	Published - 10 Dec 2019
Event	4th International Symposium of Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices, ISBE 2019 - Padang, West Sumatera, Indonesia Duration: 22 Jul 2019 → 24 Jul 2019

Publication series

Name	AIP Conference Proceedings
Volume	2193
ISSN (Print)	0094-243X
ISSN (Electronic)	1551-7616

Conference

Conference	4th International Symposium of Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices, ISBE 2019
Country/Territory	Indonesia
City	Padang, West Sumatera
Period	22/07/19 → 24/07/19

Keywords

Ceiba petranda
denv-2
Euginia uniflora
huh7it-1 cell line

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1063/1.5139340

Cite this

Dewi, B. E., Angelina, M., Ardiantara, S., Prakoso, A. R., Desti, H., & Sudiro, T. M. (2019). Antiviral activity of Ceiba pentandra and Eugenia uniflora leaf extracts to dengue virus serotype-2 in Huh 7it-1 cell line. In K. Lischer, T. Abuzairi, S. F. Rahman, & M. Gozan (Eds.), 4th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2019 Article 030003 (AIP Conference Proceedings; Vol. 2193). American Institute of Physics Inc.. https://doi.org/10.1063/1.5139340

Dewi, Beti Ernawati ; Angelina, Marissa ; Ardiantara, Sabda et al. / Antiviral activity of Ceiba pentandra and Eugenia uniflora leaf extracts to dengue virus serotype-2 in Huh 7it-1 cell line. 4th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2019. editor / Kenny Lischer ; Tomy Abuzairi ; Siti Fauziyah Rahman ; Misri Gozan. American Institute of Physics Inc., 2019. (AIP Conference Proceedings).

@inproceedings{3df33f6cca184ef38b0cd7c7511359be,

title = "Antiviral activity of Ceiba pentandra and Eugenia uniflora leaf extracts to dengue virus serotype-2 in Huh 7it-1 cell line",

abstract = "Dengue Fever (DF) is an infectious disease caused by dengue virus (DENV) infection which is transmitted by Aedes aegypti and Aedes albopictus mosquito bite. There are approximately 50 million DENV cases annually in the world with 500 thousand cases hospitalized. Incidence rate of DENV infection is increasing in Indonesia every year. Specific antiviral that can inhibit or mitigate the progression of the disease have been investigated for many decades. However, until now, management of DENV patients is only supportive. Specific antiviral drug to DENV is not available yet. The objective of this study is to determine antiviral activity of Ceiba pentandra and Eugenia uniflora leaf extract against DENV replication in Huh7it-1 cell line. Study design was an experimental study with a Focus assay and MTT assay to determine the effectivity and toxicity, respectively. Ceiba pentandra leaf extract showed IC50, CC50 and SI of 15.49 μg/ml, 81.1 μg/ml and 5.23. In other hand, E. uniflora revealed IC50, CC50, and SI of 19.83 μg/ml, 134.42 μg/ml and 6.78. We concluded that C. pentandra and E. uniflora leaf extract have potential as antiviral drug to DENV. Further studies are needed to explore the substance of C. pentandra and E. uniflora leaf extract that have antiviral activities to DENV.",

keywords = "Ceiba petranda, denv-2, Euginia uniflora, huh7it-1 cell line",

author = "Dewi, {Beti Ernawati} and Marissa Angelina and Sabda Ardiantara and Prakoso, {Agung Riyanto} and Hidayati Desti and Sudiro, {Tjahjani Mirawati}",

note = "Publisher Copyright: {\textcopyright} 2019 Author(s).; 4th International Symposium of Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices, ISBE 2019 ; Conference date: 22-07-2019 Through 24-07-2019",

year = "2019",

month = dec,

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doi = "10.1063/1.5139340",

language = "English",

series = "AIP Conference Proceedings",

publisher = "American Institute of Physics Inc.",

editor = "Kenny Lischer and Tomy Abuzairi and Rahman, {Siti Fauziyah} and Misri Gozan",

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Dewi, BE, Angelina, M, Ardiantara, S, Prakoso, AR, Desti, H & Sudiro, TM 2019, Antiviral activity of Ceiba pentandra and Eugenia uniflora leaf extracts to dengue virus serotype-2 in Huh 7it-1 cell line. in K Lischer, T Abuzairi, SF Rahman & M Gozan (eds), 4th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2019., 030003, AIP Conference Proceedings, vol. 2193, American Institute of Physics Inc., 4th International Symposium of Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices, ISBE 2019, Padang, West Sumatera, Indonesia, 22/07/19. https://doi.org/10.1063/1.5139340

Antiviral activity of Ceiba pentandra and Eugenia uniflora leaf extracts to dengue virus serotype-2 in Huh 7it-1 cell line. / Dewi, Beti Ernawati; Angelina, Marissa; Ardiantara, Sabda et al.
4th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2019. ed. / Kenny Lischer; Tomy Abuzairi; Siti Fauziyah Rahman; Misri Gozan. American Institute of Physics Inc., 2019. 030003 (AIP Conference Proceedings; Vol. 2193).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Antiviral activity of Ceiba pentandra and Eugenia uniflora leaf extracts to dengue virus serotype-2 in Huh 7it-1 cell line

AU - Dewi, Beti Ernawati

AU - Angelina, Marissa

AU - Ardiantara, Sabda

AU - Prakoso, Agung Riyanto

AU - Desti, Hidayati

AU - Sudiro, Tjahjani Mirawati

PY - 2019/12/10

Y1 - 2019/12/10

N2 - Dengue Fever (DF) is an infectious disease caused by dengue virus (DENV) infection which is transmitted by Aedes aegypti and Aedes albopictus mosquito bite. There are approximately 50 million DENV cases annually in the world with 500 thousand cases hospitalized. Incidence rate of DENV infection is increasing in Indonesia every year. Specific antiviral that can inhibit or mitigate the progression of the disease have been investigated for many decades. However, until now, management of DENV patients is only supportive. Specific antiviral drug to DENV is not available yet. The objective of this study is to determine antiviral activity of Ceiba pentandra and Eugenia uniflora leaf extract against DENV replication in Huh7it-1 cell line. Study design was an experimental study with a Focus assay and MTT assay to determine the effectivity and toxicity, respectively. Ceiba pentandra leaf extract showed IC50, CC50 and SI of 15.49 μg/ml, 81.1 μg/ml and 5.23. In other hand, E. uniflora revealed IC50, CC50, and SI of 19.83 μg/ml, 134.42 μg/ml and 6.78. We concluded that C. pentandra and E. uniflora leaf extract have potential as antiviral drug to DENV. Further studies are needed to explore the substance of C. pentandra and E. uniflora leaf extract that have antiviral activities to DENV.

AB - Dengue Fever (DF) is an infectious disease caused by dengue virus (DENV) infection which is transmitted by Aedes aegypti and Aedes albopictus mosquito bite. There are approximately 50 million DENV cases annually in the world with 500 thousand cases hospitalized. Incidence rate of DENV infection is increasing in Indonesia every year. Specific antiviral that can inhibit or mitigate the progression of the disease have been investigated for many decades. However, until now, management of DENV patients is only supportive. Specific antiviral drug to DENV is not available yet. The objective of this study is to determine antiviral activity of Ceiba pentandra and Eugenia uniflora leaf extract against DENV replication in Huh7it-1 cell line. Study design was an experimental study with a Focus assay and MTT assay to determine the effectivity and toxicity, respectively. Ceiba pentandra leaf extract showed IC50, CC50 and SI of 15.49 μg/ml, 81.1 μg/ml and 5.23. In other hand, E. uniflora revealed IC50, CC50, and SI of 19.83 μg/ml, 134.42 μg/ml and 6.78. We concluded that C. pentandra and E. uniflora leaf extract have potential as antiviral drug to DENV. Further studies are needed to explore the substance of C. pentandra and E. uniflora leaf extract that have antiviral activities to DENV.

KW - Ceiba petranda

KW - denv-2

KW - Euginia uniflora

KW - huh7it-1 cell line

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M3 - Conference contribution

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T3 - AIP Conference Proceedings

BT - 4th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices

A2 - Lischer, Kenny

A2 - Abuzairi, Tomy

A2 - Rahman, Siti Fauziyah

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PB - American Institute of Physics Inc.

T2 - 4th International Symposium of Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices, ISBE 2019

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ER -

Dewi BE, Angelina M, Ardiantara S, Prakoso AR, Desti H, Sudiro TM. Antiviral activity of Ceiba pentandra and Eugenia uniflora leaf extracts to dengue virus serotype-2 in Huh 7it-1 cell line. In Lischer K, Abuzairi T, Rahman SF, Gozan M, editors, 4th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2019. American Institute of Physics Inc. 2019. 030003. (AIP Conference Proceedings). doi: 10.1063/1.5139340

Antiviral activity of Ceiba pentandra and Eugenia uniflora leaf extracts to dengue virus serotype-2 in Huh 7it-1 cell line

Abstract

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Keywords

UN SDGs

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