TY - GEN
T1 - Antiviral activity of Ceiba pentandra and Eugenia uniflora leaf extracts to dengue virus serotype-2 in Huh 7it-1 cell line
AU - Dewi, Beti Ernawati
AU - Angelina, Marissa
AU - Ardiantara, Sabda
AU - Prakoso, Agung Riyanto
AU - Desti, Hidayati
AU - Sudiro, Tjahjani Mirawati
N1 - Publisher Copyright:
© 2019 Author(s).
PY - 2019/12/10
Y1 - 2019/12/10
N2 - Dengue Fever (DF) is an infectious disease caused by dengue virus (DENV) infection which is transmitted by Aedes aegypti and Aedes albopictus mosquito bite. There are approximately 50 million DENV cases annually in the world with 500 thousand cases hospitalized. Incidence rate of DENV infection is increasing in Indonesia every year. Specific antiviral that can inhibit or mitigate the progression of the disease have been investigated for many decades. However, until now, management of DENV patients is only supportive. Specific antiviral drug to DENV is not available yet. The objective of this study is to determine antiviral activity of Ceiba pentandra and Eugenia uniflora leaf extract against DENV replication in Huh7it-1 cell line. Study design was an experimental study with a Focus assay and MTT assay to determine the effectivity and toxicity, respectively. Ceiba pentandra leaf extract showed IC50, CC50 and SI of 15.49 μg/ml, 81.1 μg/ml and 5.23. In other hand, E. uniflora revealed IC50, CC50, and SI of 19.83 μg/ml, 134.42 μg/ml and 6.78. We concluded that C. pentandra and E. uniflora leaf extract have potential as antiviral drug to DENV. Further studies are needed to explore the substance of C. pentandra and E. uniflora leaf extract that have antiviral activities to DENV.
AB - Dengue Fever (DF) is an infectious disease caused by dengue virus (DENV) infection which is transmitted by Aedes aegypti and Aedes albopictus mosquito bite. There are approximately 50 million DENV cases annually in the world with 500 thousand cases hospitalized. Incidence rate of DENV infection is increasing in Indonesia every year. Specific antiviral that can inhibit or mitigate the progression of the disease have been investigated for many decades. However, until now, management of DENV patients is only supportive. Specific antiviral drug to DENV is not available yet. The objective of this study is to determine antiviral activity of Ceiba pentandra and Eugenia uniflora leaf extract against DENV replication in Huh7it-1 cell line. Study design was an experimental study with a Focus assay and MTT assay to determine the effectivity and toxicity, respectively. Ceiba pentandra leaf extract showed IC50, CC50 and SI of 15.49 μg/ml, 81.1 μg/ml and 5.23. In other hand, E. uniflora revealed IC50, CC50, and SI of 19.83 μg/ml, 134.42 μg/ml and 6.78. We concluded that C. pentandra and E. uniflora leaf extract have potential as antiviral drug to DENV. Further studies are needed to explore the substance of C. pentandra and E. uniflora leaf extract that have antiviral activities to DENV.
KW - Ceiba petranda
KW - denv-2
KW - Euginia uniflora
KW - huh7it-1 cell line
UR - http://www.scopus.com/inward/record.url?scp=85076741250&partnerID=8YFLogxK
U2 - 10.1063/1.5139340
DO - 10.1063/1.5139340
M3 - Conference contribution
AN - SCOPUS:85076741250
T3 - AIP Conference Proceedings
BT - 4th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices
A2 - Lischer, Kenny
A2 - Abuzairi, Tomy
A2 - Rahman, Siti Fauziyah
A2 - Gozan, Misri
PB - American Institute of Physics Inc.
T2 - 4th International Symposium of Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices, ISBE 2019
Y2 - 22 July 2019 through 24 July 2019
ER -
