TY - JOUR
T1 - Antioxidant properties of red betel (Piper crocatum) leaf extract and its compounds
AU - Lister, I. Nyoman Ehrich
AU - Ginting, Chrismis Novalinda
AU - Girsang, Ermi
AU - Armansyah, Adek
AU - Marpaung, Hans Hotma
AU - Sinaga, Ade Putra Fratama
AU - Handayani, Rr Anisa Siwianti
AU - Rizal, Rizal
N1 - Funding Information:
We gratefully acknowledge the financial and facilities support provided by the Research Institutions and Community Service of Universitas Prima Indonesia. Medan, Indonesia for research grant 2018 and also Aretha Medika Utama Biomolecular and Biomedical Research Center, Bandung, Indonesia for providing research methodology and laboratory facilities. We would also extend our gratitude to Annisa Amalia, Ubaydillah Zedd Munshy, Satrio Haryo Benowo Wibowo, Hanna Sari Widya Kusuma, Wahyu Setia Widodo from Aretha Medika Utama-Biomolecular and Biomedical Research Center, Bandung, Indonesia for their technical support.
Publisher Copyright:
© 2019, Informatics Publishing Limited. All rights reserved.
PY - 2019/10
Y1 - 2019/10
N2 - Background: Piper crocatum, also known as red betel, is a potential herbal medicine. Aims: Current study was planned to determine the antioxidant activities of red betel (Piper crocatum Ruiz and Pav.) leaves extract (RBLE) as compared to eugenol and hydroxychavicol compounds. Methods: DPPH radical scavenging, H2O2 scavenging, ABTS reduction, and FRAP reduction assay were carried out. Results: In DPPH scavenging, RBLE showed an IC50 value of 3.98 μg/mL, eugenol of 2.98 μg/mL, and hydroxychavicol of 18.00 μg/mL. Meanwhile, H2O2 scavenging activity showed an IC50 value of RBLE, eugenol, and hydroxychavicol as 186.33 μg/mL, 97.36 μg/mL, and 41.06 μg/mL respectively. ABTS reduction assay showed an IC50 value of 38.43 μg/mL, 181 μg/mL, and 3.10 μg/mL for RBLE, eugenol, and hydroxychavicol respectively. The highest FRAP reduction activity was shown by Eugenol with a concentration of 50 μg/mL which was equal to 424.67 µM Fe (II)/µg. Conclusion: The RBLE and its compounds (eugenol and hydroxychavicol) have antioxidant activity as indicated by the results of the DPPH scavenging, H2O2 scavenging, ABTS reduction, and FRAP reduction assays. However, RBLE had the lowest antioxidant activity compared to other compounds.
AB - Background: Piper crocatum, also known as red betel, is a potential herbal medicine. Aims: Current study was planned to determine the antioxidant activities of red betel (Piper crocatum Ruiz and Pav.) leaves extract (RBLE) as compared to eugenol and hydroxychavicol compounds. Methods: DPPH radical scavenging, H2O2 scavenging, ABTS reduction, and FRAP reduction assay were carried out. Results: In DPPH scavenging, RBLE showed an IC50 value of 3.98 μg/mL, eugenol of 2.98 μg/mL, and hydroxychavicol of 18.00 μg/mL. Meanwhile, H2O2 scavenging activity showed an IC50 value of RBLE, eugenol, and hydroxychavicol as 186.33 μg/mL, 97.36 μg/mL, and 41.06 μg/mL respectively. ABTS reduction assay showed an IC50 value of 38.43 μg/mL, 181 μg/mL, and 3.10 μg/mL for RBLE, eugenol, and hydroxychavicol respectively. The highest FRAP reduction activity was shown by Eugenol with a concentration of 50 μg/mL which was equal to 424.67 µM Fe (II)/µg. Conclusion: The RBLE and its compounds (eugenol and hydroxychavicol) have antioxidant activity as indicated by the results of the DPPH scavenging, H2O2 scavenging, ABTS reduction, and FRAP reduction assays. However, RBLE had the lowest antioxidant activity compared to other compounds.
KW - Eugenol
KW - Free Radicals
KW - Hydroxychavicol
KW - Piper crocatum
UR - http://www.scopus.com/inward/record.url?scp=85077704610&partnerID=8YFLogxK
U2 - 10.18311/jnr/2019/23633
DO - 10.18311/jnr/2019/23633
M3 - Article
AN - SCOPUS:85077704610
SN - 0972-5547
VL - 19
SP - 198
EP - 205
JO - Journal of Natural Remedies
JF - Journal of Natural Remedies
IS - 4
ER -