Antioxidant and cytotoxic bioassay on blumeodendron toxbrai (blume.) stem bark hexane, dichloromethane, and methanolic ekstract

Introduction Blumeodendron toksbraii has the potential to be anti-HIV and anti α-glucosidase . Objective: This research was conducted to examine the effects of antioxidant and cytotoxicity in vitro from these compounds from methanolic stem bark extract. Method: Stem bark to be extracted with maceration using hexane, dichloromethane, and methanol solution. Extracts were quantified with respect to in vitro antioxidant activity using the 2.2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging. Anticytotoxic activity was determined by cytotoxicity assay using MCF-7 cell line with Alamar Blue method. Results: The observed IC₅₀ value from hexane, dichloromethane, and methanol extract for antioxidant assay were 88.33 ± 0.19 µg/ mL, 74,54 ± 0,61 µg /mL and 94.1 ± 0.19 µg/mL respectively. IC₅₀ value of anti-cytotoxic assay from hexane extract, dichloromethane and methanol extract is 121.24 ± 0.15 µg/mL, 55 ± 0,48 µg/mL and 70.71 ± 0.15 µg/mL. Conclusion: dichloromethane extract showed good promising result for anti-oxidant and cytotoxic assay, futher study needed to isolate compound from this plant.