Antioxidant and anticancer activity of Eleutherine bulbosa (Mill.) Urb on leukemia cells L₁₂₁₀

Antioxidant and anticancer activity test on Leukemia cells L₁₂₁₀ were carried out on four fractions (ethanol extract, n-hexane, chloroform, and water fraction) of Eleutherine bulbosa [Mill.] Urb. The purpose of this research is to obtain IC₅₀ antioxidant and anticancer activity of the active fraction of Eleutherine. bulbosa [Mill.] Urb on Leukemia cells L₁₂₁₀. The research method includes antioxidant activity test with DPPH (2,2-diphenyl-1-picrylhydrazyl) method, anticancer activity test of active fraction on Leukemia cell L₁₂₁₀, and GC-MS analysis to determine the active compound. Chloroform fraction has the best antioxidant activity with IC₅₀ value 19.694 ppm (very strong category). The IC₅₀ value of the chloroform fraction on L1210 Leukemia cells was 9.56 ppm (very strong category). Based on GC-MS analysis of the chloroform fraction, some compounds such as hexadecanoic acid, 9,12-octadecadienoic acid, linolenic acid, octadecanoic acid, androstan-17-one, and 1-(2,3,5,6-tetramethylphenyl)-ethanone were detected.