TY - JOUR
T1 - Antioxidant and antibacterial assay against fish pathogen bacteria of Kjellbergiodendron celebicum (Koord.) merr. leaf extract
AU - Priscilia, Bianca
AU - Nugraha, Media Fitri Isma
AU - Novita, Hessy
AU - Elya, Berna
N1 - Funding Information:
The authors are very grateful for the research grand opportunity and funding provided by the Ministry of Research, Technology, and Higher Education of the Republic of Indonesia, Dr. Media Fitri Isma Nugraha, Insinas Grand Research Program in 2018 - 2019. We also thank Dr. Alias Radjamuddin from Pangkep State Polytechnic of Agriculture South Sulawesi, Prof (Ris). Dr. Andi Akhmad Mustafa, Dr. Andi Parenrengi, and the members of the Research Institute for Brackish water Aquaculture and Fisheries (BRPBAP3) Maros for all the support given during the survey in South Sulawesi. We are very thanks to Dr. Idil Ardi chef of Indonesian Research Institute for Ornamental Fish Culture – the Ministry Marine and Fisheries. We extend our sincere thanks to the Indonesian Research Institute for Ornamental Fish Culture – the Ministry Marine and Fisheries and Laboratory of Pharmacognosy, of the Faculty of Pharmacy of Universitas Indonesia for providing facilities.
Funding Information:
The authors are very grateful for the research grand opportunity and funding provided by the Ministry of Research, Technology, and Higher Education of the Republic of Indonesia, Dr. Media Fitri Isma Nugraha, Insinas Grand Research Program in 2018 - 2019. We also thank Dr. Alias Radjamuddin from Pangkep State Polytechnic of Agriculture South Sulawesi, Prof (Ris). Dr. Andi Akhmad Mustafa, Dr. Andi Parenrengi, and the members of the Research Institute for Brackish water Aquaculture and Fisheries (BRPBAP3) Maros for all the support given during the survey in South Sulawesi. We are very thanks to Dr. Idil Ardi chef of Indonesian Research Institute for Ornamental Fish Culture - the Ministry Marine and Fisheries. We extend our sincere thanks to the Indonesian Research Institute for Ornamental Fish Culture - the Ministry Marine and Fisheries and Laboratory of Pharmacognosy, of the Faculty of Pharmacy of Universitas Indonesia for providing facilities.
Publisher Copyright:
© 2020 Phcogj.Com.
PY - 2020/1/1
Y1 - 2020/1/1
N2 - Introduction: Kjellbergiodendron celebicum (Koord.) Merr. (local name: tombe uwa) is a plant endemic to Sulawesi, Indonesia, and grows around lakes or aquatic environments where fish live. Based on phytochemical screening in previous studies, i.e. methanol extract and ethyl acetate fraction from the leaves of Kjellbergiodendron celebicum (Koord.) Merr., the methanol extract gives positive results containing polyphenol compounds in the flavonoid group which have been known to have strong antioxidant and antibacterial properties. Objective: To test the effectiveness of the comparison of the natural content in the compounds (antibacterial and antioxidant properties) and the total content of phenol in Kjellbergiodendron celebicum (Koord.) Merr., which was extracted using two methods, i.e. maceration and Ultrasonic-Assisted Extraction (UAE), in fish-disease bacteria. Method: The leaves were separated to be extracted with two different methods: maceration and Ultrasound-Assisted Extraction (UAE). Extracts were first screened qualitatively for antioxidant activity and then quantified with respect to in vitro antioxidant activity using the 2.2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and the ferric-reducing antioxidant power (FRAP) assay. Antibacterial activity was determined by the paper disc diffusion method and microdilution. Results: 70% Ethanol in leaves extract of Kjellbergiodendron celebicum (Koord.) Merr. The extract which has the highest activity based on the DPPH test and FRAP test is the extract from UAE extraction with IC50 value of 9.81512 μg/mL and ferrous equivalent antioxidant capacity (FeEAC) value of 1.661.3 μmol/gr. UAE method also has a higher potential in antibacterial activity based on the diffusion method of paper discs and microdilution with the MIC obtained as much as 390.6 μg/mL. Conclusion: the UAE extraction method is better at scanning polyphenol compounds compared to the conventional maceration extraction method. Therefore, the results of the antioxidant and antibacterial activity using the UAE method are better than the maceration method.
AB - Introduction: Kjellbergiodendron celebicum (Koord.) Merr. (local name: tombe uwa) is a plant endemic to Sulawesi, Indonesia, and grows around lakes or aquatic environments where fish live. Based on phytochemical screening in previous studies, i.e. methanol extract and ethyl acetate fraction from the leaves of Kjellbergiodendron celebicum (Koord.) Merr., the methanol extract gives positive results containing polyphenol compounds in the flavonoid group which have been known to have strong antioxidant and antibacterial properties. Objective: To test the effectiveness of the comparison of the natural content in the compounds (antibacterial and antioxidant properties) and the total content of phenol in Kjellbergiodendron celebicum (Koord.) Merr., which was extracted using two methods, i.e. maceration and Ultrasonic-Assisted Extraction (UAE), in fish-disease bacteria. Method: The leaves were separated to be extracted with two different methods: maceration and Ultrasound-Assisted Extraction (UAE). Extracts were first screened qualitatively for antioxidant activity and then quantified with respect to in vitro antioxidant activity using the 2.2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and the ferric-reducing antioxidant power (FRAP) assay. Antibacterial activity was determined by the paper disc diffusion method and microdilution. Results: 70% Ethanol in leaves extract of Kjellbergiodendron celebicum (Koord.) Merr. The extract which has the highest activity based on the DPPH test and FRAP test is the extract from UAE extraction with IC50 value of 9.81512 μg/mL and ferrous equivalent antioxidant capacity (FeEAC) value of 1.661.3 μmol/gr. UAE method also has a higher potential in antibacterial activity based on the diffusion method of paper discs and microdilution with the MIC obtained as much as 390.6 μg/mL. Conclusion: the UAE extraction method is better at scanning polyphenol compounds compared to the conventional maceration extraction method. Therefore, the results of the antioxidant and antibacterial activity using the UAE method are better than the maceration method.
KW - Aeromonas hydrophila
KW - Edwardsiella ictaluri
KW - Flavobacterium columnare
KW - Maceration
KW - Phytochemical compound
KW - Ultrasonic-assisted extraction
UR - http://www.scopus.com/inward/record.url?scp=85081543032&partnerID=8YFLogxK
U2 - 10.5530/pj.2020.12.26
DO - 10.5530/pj.2020.12.26
M3 - Article
AN - SCOPUS:85081543032
VL - 12
SP - 173
EP - 179
JO - Pharmacognosy Journal
JF - Pharmacognosy Journal
SN - 0975-3575
IS - 1
ER -