TY - GEN
T1 - Antioxidant Activity of Green Tea Extract and Myricetin
AU - Priyandoko, Didik
AU - Widowati, Wahyu
AU - Kusuma, Hanna Sari Widya
AU - Afifah, Ervi
AU - Wijayanti, Cahyaning Riski
AU - Wahyuni, Cintani Dewi
AU - Idris, Amannah Mutmainnah
AU - Putdayani, Rizka Amelia
AU - Rizal, Rizal
N1 - Funding Information:
ACKNOWLEDGMENT We gratefully acknowledge the financial support of Penelitian Dasar Unggulan Perguruan Tinggi 2020 from Direktorat General of Higher Education, Ministry of Research, Technology and Higher Education of the Republic of Indonesia (No: 10/AMD/E1/KP.PTNBH/2020). This study was supported by Aretha Medika Utama, Biomolecular and Biomedical Research Center, Bandung, Indonesia and PT.FAST, Cisalak, Indonesia. We would also extend our gratitude to Seila Arumwardana, Muhammad Aldi Maulana, Aditya Rinaldy from Aretha Medika Utama-Biomolecular and Biomedical Research Center, Bandung, Indonesia also apt. Fadhlina Fajrin, S. Farm and H. Maheda Dwinarendra, MBA from PT.FAST for their technical support.
Publisher Copyright:
© 2021 IEEE.
PY - 2021/7/14
Y1 - 2021/7/14
N2 - Free radicals are the major cause of oxidative stress, which can lead to inflammatory diseases, ischemic diseases, hemochromatosis, emphysema, and other illnesses. Antioxidant intake is essential for keeping the body's oxidative processes in balance. Green tea containt a natural antioxidant that is commonly consumed and readily available. Myricetin is a pure component of green tea. This study aims was to determine the antioxidant activity of green tea extract (GTE) compared with myricetin (MYR). Antioxidant activity properties were determined by 2,2-diphenyl-1 picrylhydrazyl (DPPH), 2,2 ′ -Azinobis(3-Ethylbenzthiazoline-6-Sulfonate) (ABTS), hydrogen peroxide (H2O2), NO scavenging, and ferric reducing antioxidant power (FRAP). The analysis results showed that the IC50 of GTE (24.00 μg/mL) was higher than MYR (4.68 μg/mL) in DPPH scavenging activity. The highest concentration of FRAP activity of GTE and MYR were 595.78±19.35 and 530.67±10.97 μM Fe (II)/μg. GTE also had a higher IC50 value for ABTS reducing activity than MYR, at 25.16 μg/mL versus 16.78 μg/mL. According to H2O2 scavenging activity, MYR has higher antioxidant activity (133.32 μg/mL) than GTE (137.31 μg/mL). Meanwhile, the NO scavenging assay results show that MYR (19.70 μg/mL) has a higher IC50 value than GTE (7.10 μg/mL). In conclusion, GTE has potential antioxidant activity like its particular compound, MYR.
AB - Free radicals are the major cause of oxidative stress, which can lead to inflammatory diseases, ischemic diseases, hemochromatosis, emphysema, and other illnesses. Antioxidant intake is essential for keeping the body's oxidative processes in balance. Green tea containt a natural antioxidant that is commonly consumed and readily available. Myricetin is a pure component of green tea. This study aims was to determine the antioxidant activity of green tea extract (GTE) compared with myricetin (MYR). Antioxidant activity properties were determined by 2,2-diphenyl-1 picrylhydrazyl (DPPH), 2,2 ′ -Azinobis(3-Ethylbenzthiazoline-6-Sulfonate) (ABTS), hydrogen peroxide (H2O2), NO scavenging, and ferric reducing antioxidant power (FRAP). The analysis results showed that the IC50 of GTE (24.00 μg/mL) was higher than MYR (4.68 μg/mL) in DPPH scavenging activity. The highest concentration of FRAP activity of GTE and MYR were 595.78±19.35 and 530.67±10.97 μM Fe (II)/μg. GTE also had a higher IC50 value for ABTS reducing activity than MYR, at 25.16 μg/mL versus 16.78 μg/mL. According to H2O2 scavenging activity, MYR has higher antioxidant activity (133.32 μg/mL) than GTE (137.31 μg/mL). Meanwhile, the NO scavenging assay results show that MYR (19.70 μg/mL) has a higher IC50 value than GTE (7.10 μg/mL). In conclusion, GTE has potential antioxidant activity like its particular compound, MYR.
KW - antioxidants
KW - free radicals
KW - green tea
KW - myricetin
KW - oxidative stress
UR - http://www.scopus.com/inward/record.url?scp=85115984796&partnerID=8YFLogxK
U2 - 10.1109/InHeNce52833.2021.9537285
DO - 10.1109/InHeNce52833.2021.9537285
M3 - Conference contribution
AN - SCOPUS:85115984796
T3 - InHeNce 2021 - 2021 IEEE International Conference on Health, Instrumentation and Measurement, and Natural Sciences
BT - InHeNce 2021 - 2021 IEEE International Conference on Health, Instrumentation and Measurement, and Natural Sciences
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 2021 IEEE International Conference on Health, Instrumentation and Measurement, and Natural Sciences, InHeNce 2021
Y2 - 14 July 2021 through 16 July 2021
ER -