TY - JOUR
T1 - Antioxidant activity of endophytic fungi culture extracts of Christ’s thorn jujube (Ziziphus spina-christi)
AU - Agusta, Andria
AU - Lestari, Hanna Puji
AU - Ridwan, Rahmawati
AU - Ilyas, Muhammad
AU - Evana, Evana
AU - Praptiwi, Praptiwi
N1 - Funding Information:
The authors would like to thank the Research Institute for Spices and Medicinal Plants, Bogor, for providing the plant sample Christ’s thorn jujube (Z. spina-christi Lam. Desf.). Thanks are also expressed to Andi Saptaji Kamal for his support in the laboratory. There is no a special fund for this research.
Publisher Copyright:
© 2022 Marmara University Press ISSN: 2630-6344.
PY - 2022
Y1 - 2022
N2 - This study aims to explore 50 extracts from fungal endophytes from roots, stems, and leaves of Z. spina-christi based on the chemical content and their antioxidant activity. Isolation of fungal endophytes was carried out in CMMA and PDA media. Cultivation was in PDB and GYP media. Determination of antioxidant activity qualitatively by TLC-bioautography and quantitatively by DPPH method. Phytochemical screening of these extracts revealed the presence of alkaloids, phenolics, flavonoids, and tannins. The results showed that seven types of fungal endophytes had antioxidant activity, namely Trichoderma sp., Aspergillus sect. Flavi, Aspergillus sect. Nigri, Aspergillus sect. Terrei, Phomopsis sp., Sordariomycetes, and Hyphomycetes. One root isolate from PDB (Aspergillus sect. Terrei Bi. Ak. 3.3) possesses very strong antioxidant activity (IC50 of 9.17 µg/mL and AAI = 3.35) and one stem isolate (Aspergillus nigri Bi. Bt. 1.2.2) has strong antioxidant activity (IC50 22.13 µg/mL and AAI = 1.38). The fungal endophyte culture extract from GYP media produced one root isolate (Aspergillus terrei Bi. Ak. 3.3) with strong antioxidant activity (IC50 19.54 µg/mL and AAI = 1.57). It can be concluded that the fungal endophytes of Z. spina-christi have the potential to be developed as an antioxidant.
AB - This study aims to explore 50 extracts from fungal endophytes from roots, stems, and leaves of Z. spina-christi based on the chemical content and their antioxidant activity. Isolation of fungal endophytes was carried out in CMMA and PDA media. Cultivation was in PDB and GYP media. Determination of antioxidant activity qualitatively by TLC-bioautography and quantitatively by DPPH method. Phytochemical screening of these extracts revealed the presence of alkaloids, phenolics, flavonoids, and tannins. The results showed that seven types of fungal endophytes had antioxidant activity, namely Trichoderma sp., Aspergillus sect. Flavi, Aspergillus sect. Nigri, Aspergillus sect. Terrei, Phomopsis sp., Sordariomycetes, and Hyphomycetes. One root isolate from PDB (Aspergillus sect. Terrei Bi. Ak. 3.3) possesses very strong antioxidant activity (IC50 of 9.17 µg/mL and AAI = 3.35) and one stem isolate (Aspergillus nigri Bi. Bt. 1.2.2) has strong antioxidant activity (IC50 22.13 µg/mL and AAI = 1.38). The fungal endophyte culture extract from GYP media produced one root isolate (Aspergillus terrei Bi. Ak. 3.3) with strong antioxidant activity (IC50 19.54 µg/mL and AAI = 1.57). It can be concluded that the fungal endophytes of Z. spina-christi have the potential to be developed as an antioxidant.
KW - antioxidant
KW - Christ's thorn jujube
KW - fungal endophytes
KW - Ziziphus spina-christi Lam. Desf
UR - http://www.scopus.com/inward/record.url?scp=85142147662&partnerID=8YFLogxK
U2 - 10.29228/jrp.266
DO - 10.29228/jrp.266
M3 - Article
AN - SCOPUS:85142147662
SN - 1309-0801
VL - 26
SP - 1758
EP - 1770
JO - Journal of Research in Pharmacy
JF - Journal of Research in Pharmacy
IS - 6
ER -