Objective: This study aimed to fractionate the antioxidant activity of the ethyl acetate leaf extract and to characterize the most active fractions according to compound groups. Methods: The ethyl acetate extract was fractionated with column chromatography using a gradient elution system. Fractions were first screened qualitatively for antioxidant activity before active fractions were quantified with respect to in vitro antioxidant activity using the 2,2-diphenyl-1- picrylhydrazyl (DPPH) radical scavenging assay and the ferric-reducing antioxidant power (FRAP) assay. The compound groups were identified following separation by thin-layer chromatography. Results: Fraction 11 exhibited the greatest DPPH radical-scavenging activity, with an IC50 value of 6.58 µg/mL, while the fraction with the greatest antioxidant activity according to the FRAP assay was fraction 10, with a ferric ion equivalent antioxidant activity value of 1015.34 µmol/g. Conclusion: Compound group identification revealed that Fractions 10 and 11 contained flavonoids, with two common to both fractions, while fraction 10 also contained one specific flavonoid.
- Antioxidant; 2,2-Diphenyl-1-picrylhydrazyl
- Ferric-reducing antioxidant power
- Garcinia fruticosa