TY - JOUR
T1 - Antimicrobial Compound from Endophytic Pseudomonas azotoformans UICC B-91 of Neesia altissima (Malvaceae)
AU - Pratiwi, Rina Hidayati
AU - Oktarina, Eva
AU - Mangunwardoyo, Wibowo
AU - Hidayat, Iman
AU - Saepudin, Endang
N1 - Funding Information:
This research was supported by Research Grant of DRPM Kemenristek BRIN-Kemendikbud Ristek-LLDIKTI3 awarded to RHP number 1867/E4/AK.04/2021. The authors acknowledge the facilities, scientific and technical support from Advanced Characterization Laboratories Serpong, National Research and Innovation Institute through E-Layanan Sains, Badan Riset dan Inovasi Nasional. We also would like to thank to Microbiology Laboratory at Center of Chemical and Packaging, Jakarta and Research Center for Biomaterial, Indonesian Institute of Sciences (LIPI), Cibinong for valuable technical assistance.
Publisher Copyright:
© 2022 Phcogj.Com. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
PY - 2022/1
Y1 - 2022/1
N2 - Centrifugated extract of endophytic Pseudomonas azotoformans strain UICC B-91 has been observed for its antimicrobial activity against Escherichia coli ATCC 8739, Bacillus cereus ATCC 10876, Staphylococcus aureus ATCC 6583, Staphylococcus aureus ATCC 25923, Salmonella typhimurium ATCC 25241, Pseudomonas aeruginosa ATCC 15442, Bacillus subtilis ATCC 19659 and Candida albicans ATCC 10231, with minimum concentration in the range 250 µg/mL. But, the novel compound of the bioactive substances which relevant for antimicrobial activity have not been described. Further fractionation and identification of the extract by thin layer chromatography (TLC) and liquid chromatography mass spectrophotometry (LCMS-MS) detected several compound as futoamide (C18H23NO3), gentialutine (C9H11NO), gentiabetine (C9H11NO2), 1-[(2E,4E)-2,4-decadienoyl]pyrrolidine (C14H23NO), lycopodine (C16H25NO) and dihydrolycopodine (C16H27NO). The majority compounds containing CHON elements with molecular weight approximately 100-450 Da.
AB - Centrifugated extract of endophytic Pseudomonas azotoformans strain UICC B-91 has been observed for its antimicrobial activity against Escherichia coli ATCC 8739, Bacillus cereus ATCC 10876, Staphylococcus aureus ATCC 6583, Staphylococcus aureus ATCC 25923, Salmonella typhimurium ATCC 25241, Pseudomonas aeruginosa ATCC 15442, Bacillus subtilis ATCC 19659 and Candida albicans ATCC 10231, with minimum concentration in the range 250 µg/mL. But, the novel compound of the bioactive substances which relevant for antimicrobial activity have not been described. Further fractionation and identification of the extract by thin layer chromatography (TLC) and liquid chromatography mass spectrophotometry (LCMS-MS) detected several compound as futoamide (C18H23NO3), gentialutine (C9H11NO), gentiabetine (C9H11NO2), 1-[(2E,4E)-2,4-decadienoyl]pyrrolidine (C14H23NO), lycopodine (C16H25NO) and dihydrolycopodine (C16H27NO). The majority compounds containing CHON elements with molecular weight approximately 100-450 Da.
KW - Alkaloid
KW - Antimicrobial compound
KW - Extraction
KW - P. azotoformans
UR - http://www.scopus.com/inward/record.url?scp=85125733545&partnerID=8YFLogxK
U2 - 10.5530/pj.2022.14.23
DO - 10.5530/pj.2022.14.23
M3 - Article
AN - SCOPUS:85125733545
SN - 0975-3575
VL - 14
SP - 172
EP - 181
JO - Pharmacognosy Journal
JF - Pharmacognosy Journal
IS - 1
ER -