Antimicrobial activity of lichens-associated actinomycetes strain LC-23

Agustina E. Susanti; Shanti Ratnakomala; W. Mangunwardoyo; Puspita Lisdiyanti

doi:10.1145/3314367.3314386

Antimicrobial activity of lichens-associated actinomycetes strain LC-23

Agustina E. Susanti, Shanti Ratnakomala, W. Mangunwardoyo, Puspita Lisdiyanti

Department of Biology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

2 Citations (Scopus)

Abstract

Actinomycetes known as the largest antibiotic producer that has a broad range habitat. Research has been done to find a new antibiotic from the various habitats of actinomycetes. Lichens was the symbiotic structure of alga and fungi known as the ecological niche of various kinds of microbes including actinomycetes. The aim of this study in deep of isolate strain LC-23 for production of antimicrobial, characterize the metabolite profile of the ethyl acetate extract and identification based on 16S rRNA gene sequence analysis. The result revealed actinomycetes from lichen, strain LC-23 showed potency against Gram positive bacteria. Ethyl acetate extract of the strain showed positive inhibition against Staphylococcus aureus BTCC B-611 and Micrococcus luteus BTCC B-552. Minimum Inhibitory Concentration (MIC) of extract LC-23 was less than 2.106 ppm. The ethyl acetate extract was subjected to fractionation and tested against the pathogenic microbes and showed inhibition activity in column 9 and 10, and specifically in column 7E to 7G for Staphylcoccus aureus BTCC B-611 and 7E-7H for Micrococcus luteus BTCC B-552. The identification based on 16S rRNA gene sequence showed strain LC-23 was 98.51% similarity to Streptomyces palmae type strain. Neighbor-joining phylogenetic tree confirmed the relationships of this strain to other members of Streptomyces genera.

Original language	English
Title of host publication	ICBBB 2019 - 2019 9th International Conference on Bioscience, Biochemistry and Bioinformatics
Publisher	Association for Computing Machinery
Pages	91-96
Number of pages	6
ISBN (Electronic)	9781450366540
DOIs	https://doi.org/10.1145/3314367.3314386
Publication status	Published - 7 Jan 2019
Event	9th International Conference on Bioscience, Biochemistry and Bioinformatics, ICBBB 2019 - Singapore, Singapore Duration: 7 Jan 2019 → 9 Jan 2019

Publication series

Name	ACM International Conference Proceeding Series

Conference

Conference	9th International Conference on Bioscience, Biochemistry and Bioinformatics, ICBBB 2019
Country/Territory	Singapore
City	Singapore
Period	7/01/19 → 9/01/19

Keywords

Actinomycetes
Antimicrobial
Identification 16S srRNA gene
Lichens

Access to Document

10.1145/3314367.3314386

Cite this

Susanti, A. E., Ratnakomala, S., Mangunwardoyo, W., & Lisdiyanti, P. (2019). Antimicrobial activity of lichens-associated actinomycetes strain LC-23. In ICBBB 2019 - 2019 9th International Conference on Bioscience, Biochemistry and Bioinformatics (pp. 91-96). (ACM International Conference Proceeding Series). Association for Computing Machinery. https://doi.org/10.1145/3314367.3314386

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title = "Antimicrobial activity of lichens-associated actinomycetes strain LC-23",

abstract = "Actinomycetes known as the largest antibiotic producer that has a broad range habitat. Research has been done to find a new antibiotic from the various habitats of actinomycetes. Lichens was the symbiotic structure of alga and fungi known as the ecological niche of various kinds of microbes including actinomycetes. The aim of this study in deep of isolate strain LC-23 for production of antimicrobial, characterize the metabolite profile of the ethyl acetate extract and identification based on 16S rRNA gene sequence analysis. The result revealed actinomycetes from lichen, strain LC-23 showed potency against Gram positive bacteria. Ethyl acetate extract of the strain showed positive inhibition against Staphylococcus aureus BTCC B-611 and Micrococcus luteus BTCC B-552. Minimum Inhibitory Concentration (MIC) of extract LC-23 was less than 2.106 ppm. The ethyl acetate extract was subjected to fractionation and tested against the pathogenic microbes and showed inhibition activity in column 9 and 10, and specifically in column 7E to 7G for Staphylcoccus aureus BTCC B-611 and 7E-7H for Micrococcus luteus BTCC B-552. The identification based on 16S rRNA gene sequence showed strain LC-23 was 98.51% similarity to Streptomyces palmae type strain. Neighbor-joining phylogenetic tree confirmed the relationships of this strain to other members of Streptomyces genera.",

keywords = "Actinomycetes, Antimicrobial, Identification 16S srRNA gene, Lichens",

author = "Susanti, {Agustina E.} and Shanti Ratnakomala and W. Mangunwardoyo and Puspita Lisdiyanti",

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year = "2019",

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doi = "10.1145/3314367.3314386",

language = "English",

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Susanti, AE, Ratnakomala, S, Mangunwardoyo, W & Lisdiyanti, P 2019, Antimicrobial activity of lichens-associated actinomycetes strain LC-23. in ICBBB 2019 - 2019 9th International Conference on Bioscience, Biochemistry and Bioinformatics. ACM International Conference Proceeding Series, Association for Computing Machinery, pp. 91-96, 9th International Conference on Bioscience, Biochemistry and Bioinformatics, ICBBB 2019, Singapore, Singapore, 7/01/19. https://doi.org/10.1145/3314367.3314386

Antimicrobial activity of lichens-associated actinomycetes strain LC-23. / Susanti, Agustina E.; Ratnakomala, Shanti; Mangunwardoyo, W. et al.
ICBBB 2019 - 2019 9th International Conference on Bioscience, Biochemistry and Bioinformatics. Association for Computing Machinery, 2019. p. 91-96 (ACM International Conference Proceeding Series).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Antimicrobial activity of lichens-associated actinomycetes strain LC-23

AU - Susanti, Agustina E.

AU - Ratnakomala, Shanti

AU - Mangunwardoyo, W.

AU - Lisdiyanti, Puspita

PY - 2019/1/7

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N2 - Actinomycetes known as the largest antibiotic producer that has a broad range habitat. Research has been done to find a new antibiotic from the various habitats of actinomycetes. Lichens was the symbiotic structure of alga and fungi known as the ecological niche of various kinds of microbes including actinomycetes. The aim of this study in deep of isolate strain LC-23 for production of antimicrobial, characterize the metabolite profile of the ethyl acetate extract and identification based on 16S rRNA gene sequence analysis. The result revealed actinomycetes from lichen, strain LC-23 showed potency against Gram positive bacteria. Ethyl acetate extract of the strain showed positive inhibition against Staphylococcus aureus BTCC B-611 and Micrococcus luteus BTCC B-552. Minimum Inhibitory Concentration (MIC) of extract LC-23 was less than 2.106 ppm. The ethyl acetate extract was subjected to fractionation and tested against the pathogenic microbes and showed inhibition activity in column 9 and 10, and specifically in column 7E to 7G for Staphylcoccus aureus BTCC B-611 and 7E-7H for Micrococcus luteus BTCC B-552. The identification based on 16S rRNA gene sequence showed strain LC-23 was 98.51% similarity to Streptomyces palmae type strain. Neighbor-joining phylogenetic tree confirmed the relationships of this strain to other members of Streptomyces genera.

AB - Actinomycetes known as the largest antibiotic producer that has a broad range habitat. Research has been done to find a new antibiotic from the various habitats of actinomycetes. Lichens was the symbiotic structure of alga and fungi known as the ecological niche of various kinds of microbes including actinomycetes. The aim of this study in deep of isolate strain LC-23 for production of antimicrobial, characterize the metabolite profile of the ethyl acetate extract and identification based on 16S rRNA gene sequence analysis. The result revealed actinomycetes from lichen, strain LC-23 showed potency against Gram positive bacteria. Ethyl acetate extract of the strain showed positive inhibition against Staphylococcus aureus BTCC B-611 and Micrococcus luteus BTCC B-552. Minimum Inhibitory Concentration (MIC) of extract LC-23 was less than 2.106 ppm. The ethyl acetate extract was subjected to fractionation and tested against the pathogenic microbes and showed inhibition activity in column 9 and 10, and specifically in column 7E to 7G for Staphylcoccus aureus BTCC B-611 and 7E-7H for Micrococcus luteus BTCC B-552. The identification based on 16S rRNA gene sequence showed strain LC-23 was 98.51% similarity to Streptomyces palmae type strain. Neighbor-joining phylogenetic tree confirmed the relationships of this strain to other members of Streptomyces genera.

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DO - 10.1145/3314367.3314386

M3 - Conference contribution

AN - SCOPUS:85065239325

T3 - ACM International Conference Proceeding Series

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Y2 - 7 January 2019 through 9 January 2019

ER -

Antimicrobial activity of lichens-associated actinomycetes strain LC-23

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Keywords

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