Abstract
Pseudomonas azotoformans UICC B-91 is known to have antimicrobial potential. The purpose of this study was to determine the inhibition mechanism of the metabolites of P. azotoformans UICC B-91 on the activity of Trichophyton mentagrophytes through morphological observations. The results showed that the metabolites produced by P. azotoformans UICC B-91 were inhibited growth of T. mentagrophytes. The zone of inhibition produced by the extract solution of P. azotoformans UICC B-91 against T. mentagrophytes at concentrations of 100 mg/mL and 80 mg/mL showed strong inhibitory power, with average values of 17.82 ± 0.32 mm and 10.79 ± 0.74 mm, respectively. The microscopic morphology of T. mentagrophytes after treatment with 0.1 g of ethyl acetate extract of P. azotoformans UICC B-91 showed wider hyphae compared to the control hyphae; abnormal cytoplasm; and granular structures between the hyphae, attached to the lateral side of the hyphae, and between the cell wall and the cell membrane. The mechanism of inhibition of the metabolites from the ethyl acetate extract of P. azotoformans UICC B-91 against T. mentagrophytes is thought to involve interference with cell walls and the function of the cell membrane.
| Original language | English |
|---|---|
| Article number | 012077 |
| Journal | IOP Conference Series: Earth and Environmental Science |
| Volume | 1271 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2023 |
| Event | 5th International Conference on Biosciences, ICOBIO 2023 - Hybrid, Bogor, Indonesia Duration: 2 Aug 2023 → 3 Aug 2023 |
Keywords
- antifungal
- inhibition mechanism
- P. azotoformans
- T. mentagrophytes