Antidiabetic activity test by inhibition of α-glucosidaseand phytochemical screening from the most active fraction of buni (Antidesma bunius L.) stem barks and leaves

Berna Elya, Amarila Malik, Purwa Indah Septi Mahanani, Bianca Loranza

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Fractions of 80% ethanol (EtOH) extract from buni (Antidesma bunius L.) stem barks and leaves were evaluated by α-glucosidase inhibitory activity.Ethyl acetate(EtOAc) fraction of buni stem barksand methanol(MeOH) fraction of buni leaves showed the highestα-glucosidase inhibitory activity with IC50 values 5.73 and 8.04 ppm. Phytochemical analysis ofethyl acetate fraction of buni stem barks displayed the presence of sugars, terpenes, and flavonoids, while MeOH fraction of buni leaves contains sugars, saponins, flavonoids, and tannins.Thosewere fractionated using vacuum liquid chromatography and combined based on TLC profiles. The result showed that G subfraction (EtOAc: MeOH (180:20) fraction) of buni stem barks and F subfraction(EtOAc: MeOH (60:140), (40:160), (20:180), (0:200)) of buni leaves are the most active with IC50 values 1.16 and 4.79 ppm. Phytochemical screening indicated the presence of sugars, and flavonoids in both G subfraction and Fsubfraction. Kinetic analyses both of simplisias confirmed that the inhibition of hydrolysis is competitive mode.

Original languageEnglish
Pages (from-to)1667-1671
Number of pages5
JournalInternational Journal of PharmTech Research
Volume4
Issue number4
Publication statusPublished - 1 Oct 2012

Keywords

  • α-glucosidase
  • Buni
  • Leaves
  • Stem bark

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