Fractions of 80% ethanol (EtOH) extract from buni (Antidesma bunius L.) stem barks and leaves were evaluated by α-glucosidase inhibitory activity.Ethyl acetate(EtOAc) fraction of buni stem barksand methanol(MeOH) fraction of buni leaves showed the highestα-glucosidase inhibitory activity with IC50 values 5.73 and 8.04 ppm. Phytochemical analysis ofethyl acetate fraction of buni stem barks displayed the presence of sugars, terpenes, and flavonoids, while MeOH fraction of buni leaves contains sugars, saponins, flavonoids, and tannins.Thosewere fractionated using vacuum liquid chromatography and combined based on TLC profiles. The result showed that G subfraction (EtOAc: MeOH (180:20) fraction) of buni stem barks and F subfraction(EtOAc: MeOH (60:140), (40:160), (20:180), (0:200)) of buni leaves are the most active with IC50 values 1.16 and 4.79 ppm. Phytochemical screening indicated the presence of sugars, and flavonoids in both G subfraction and Fsubfraction. Kinetic analyses both of simplisias confirmed that the inhibition of hydrolysis is competitive mode.
|Number of pages||5|
|Journal||International Journal of PharmTech Research|
|Publication status||Published - Oct 2012|
- Stem bark