Active compounds of plants have potency as antidiabetic that can be used in diabetes mellitus treatment. One of the strategies is maintaining postprandial glucose level through inhibition of α-amylase and α-glucosidase and preventing the hydrolysis of Glucagon Like Peptide-1 (GLP-1) through inhibition of Dipeptidyl peptidase IV. So that pre-prandial and post-prandial glucose levels can be controlled properly. The aim of this study was to determine in vitro inhibitory activity of α-glucosidase, α-amylase and Dipeptidyl peptidase IV from 42 ethanolic extracts of selected Indonesian plants. Inhibitory activity was measured using spectrophotometric method. α-amylase activity was measured at λ = 540 nm, α-glucosidase activity and the DPP-IV activity were measured at λ = 405 nm. Eight extracts inhibit both α-glucosidase and α-amylase better than acarbose as a positive control. These extracts are Camelia sinensis (white, green, fermented), Pometia pinnata, Syzygium polyanthum, Artocarpus heteropyllus, Lagerstroemia speciosa and Persea Americana. The ethanolic extracts have DPP-IV inhibitory activity between 10-20%, lower than Diprotin as positive control, except Camelia sinensis (white) which gave the inhibition more than 30% at 62.5 μg mL-1. This study showed us that some extracts have inhibition activity of α-glucosidase, α-amylase and DPP-IV.
- Diabetes mellitus
- Dipeptidyl peptidase IV