TY - JOUR
T1 - Antidiabetic activity and phytochemical screening of extracts from indonesian plants by inhibition of alpha amylase, alpha glucosidase and dipeptidyl peptidase IV
AU - Elya, Berna
AU - Handayani, Rosita
AU - Sauriasari, Rani
AU - Azizahwati, null
AU - Hasyyati, Uqie Shabrina
AU - Permana, Idam Titis
AU - Permatasari, Yunita Indah
N1 - Publisher Copyright:
© 2015 Asian Network for Scientific Information.
PY - 2015
Y1 - 2015
N2 - Active compounds of plants have potency as antidiabetic that can be used in diabetes mellitus treatment. One of the strategies is maintaining postprandial glucose level through inhibition of α-amylase and α-glucosidase and preventing the hydrolysis of Glucagon Like Peptide-1 (GLP-1) through inhibition of Dipeptidyl peptidase IV. So that pre-prandial and post-prandial glucose levels can be controlled properly. The aim of this study was to determine in vitro inhibitory activity of α-glucosidase, α-amylase and Dipeptidyl peptidase IV from 42 ethanolic extracts of selected Indonesian plants. Inhibitory activity was measured using spectrophotometric method. α-amylase activity was measured at λ = 540 nm, α-glucosidase activity and the DPP-IV activity were measured at λ = 405 nm. Eight extracts inhibit both α-glucosidase and α-amylase better than acarbose as a positive control. These extracts are Camelia sinensis (white, green, fermented), Pometia pinnata, Syzygium polyanthum, Artocarpus heteropyllus, Lagerstroemia speciosa and Persea Americana. The ethanolic extracts have DPP-IV inhibitory activity between 10-20%, lower than Diprotin as positive control, except Camelia sinensis (white) which gave the inhibition more than 30% at 62.5 μg mL-1. This study showed us that some extracts have inhibition activity of α-glucosidase, α-amylase and DPP-IV.
AB - Active compounds of plants have potency as antidiabetic that can be used in diabetes mellitus treatment. One of the strategies is maintaining postprandial glucose level through inhibition of α-amylase and α-glucosidase and preventing the hydrolysis of Glucagon Like Peptide-1 (GLP-1) through inhibition of Dipeptidyl peptidase IV. So that pre-prandial and post-prandial glucose levels can be controlled properly. The aim of this study was to determine in vitro inhibitory activity of α-glucosidase, α-amylase and Dipeptidyl peptidase IV from 42 ethanolic extracts of selected Indonesian plants. Inhibitory activity was measured using spectrophotometric method. α-amylase activity was measured at λ = 540 nm, α-glucosidase activity and the DPP-IV activity were measured at λ = 405 nm. Eight extracts inhibit both α-glucosidase and α-amylase better than acarbose as a positive control. These extracts are Camelia sinensis (white, green, fermented), Pometia pinnata, Syzygium polyanthum, Artocarpus heteropyllus, Lagerstroemia speciosa and Persea Americana. The ethanolic extracts have DPP-IV inhibitory activity between 10-20%, lower than Diprotin as positive control, except Camelia sinensis (white) which gave the inhibition more than 30% at 62.5 μg mL-1. This study showed us that some extracts have inhibition activity of α-glucosidase, α-amylase and DPP-IV.
KW - Diabetes mellitus
KW - Dipeptidyl peptidase IV
KW - α-amylase
KW - α-glucosidase
UR - http://www.scopus.com/inward/record.url?scp=84944453266&partnerID=8YFLogxK
U2 - 10.3923/pjbs.2015.279.284
DO - 10.3923/pjbs.2015.279.284
M3 - Article
AN - SCOPUS:84944453266
SN - 1028-8880
VL - 18
SP - 273
EP - 278
JO - Pakistan Journal of Biological Sciences
JF - Pakistan Journal of Biological Sciences
IS - 6
ER -