Anticancer Potency of Methanol Extract from Terminalia catappa Leaves Using In Vitro and In Silico Methods

Siti Warnasih, Ade Heri Mulyati, Diana Widiastuti, Alita Chasanah Zahra, Purwantiningsih Sugita, Laksmi Ambarsari, Hanhan Dianhar, Dyah Utami Cahyaning Rahayu

Research output: Contribution to journalArticlepeer-review

Abstract

Terminalia catappa is a plant with potential for application in various antioxidant, anti-inflammatory, antimetastatic and antitumor treatments. Therefore, this study aimed to investigate anticancer properties of methanol extract derived from T. catappa leaves, using both in vitro and in silico methods. The results showed that methanol extract had inhibition of HeLa and DU145 cell lines, with respective IC50 values of 352.50 and 954.99 μg/mL. LC-MS/MS analysis identified several active compounds within the extract, including catechin, quercetin, rutin, hirsutrin, loliolide, sesquiterpene, kaempferol, apigenin, cirsiliol, cirsimaritin and demethoxycurcumin, all of which had promising anticancer potential. These compounds performed well in the in silico test. According to computational predictions, rutin (−9.1 kcal/mol), catechin (−8.0 kcal/mol) and sesquiterpene (−8.2 kcal/mol) had higher binding affinity values than cisplatin and paclitaxel (−7.5 kcal/mol). Considering these results, the compounds were potentially anticancer agents through the inhibition of the Bcl-2 protein. Finally, T. catappa active compounds can be used as alternative candidates as a chemopreventive agent.

Original languageEnglish
Article number8057
JournalTrends in Sciences
Volume21
Issue number9
DOIs
Publication statusPublished - Sept 2024

Keywords

  • Antiproliferative
  • Bcl-2
  • DU145 cell
  • HeLa cell
  • In silico
  • LC-MS/MS
  • T. catappa

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